Effect of encapsulation on development of mouse pronuclear stage embryos in vitro

Pronuclear stage embryos with intact (ZI), slit (ZS) or completely removed (ZF) zona pellucida were encapsulated with an artificial zona pellucida (AZP) made of 1.5% sodium alginate. Embryos were cultured in KSOM medium with or without protein and their development in vitro to the blastocyst stage w...

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Bibliographic Details
Published in:Animal reproduction science 1997-08, Vol.48 (2), p.317-324
Main Authors: Elsheikh, Adil Salim, Takahashi, Yoshiyuki, Hishinuma, Mitsugu, Nour, Mohammed Sayed Mohammed, Kanagawa, Hiroshi
Format: Article
Language:English
Subjects:
Alginates
Analysis of Variance
Animals
Artificial zona pellucida
Biocompatible Materials
Cell contacts
Culture Media - chemistry
Embryonic and Fetal Development - physiology
Female
Glucuronic Acid
Hexuronic Acids
Mice
Mice, Inbred ICR
Micromanipulation
Mouse
Proteins - pharmacology
Zona Pellucida
Zygote Intrafallopian Transfer - methods
Zygote Intrafallopian Transfer - veterinary
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Summary:Pronuclear stage embryos with intact (ZI), slit (ZS) or completely removed (ZF) zona pellucida were encapsulated with an artificial zona pellucida (AZP) made of 1.5% sodium alginate. Embryos were cultured in KSOM medium with or without protein and their development in vitro to the blastocyst stage was recorded. AZP significantly ( P < 0.05) improved the development of embryos to the blastocyst stage regardless of the presence of the natural zona pellucida. The encapsulated embryos developed at a higher rate ( P < 0.05) in the absence of protein as compared with non-encapsulated embryos. Furthermore, the cell contacts at the 4-cell stage were significantly improved ( P < 0.05) with encapsulation. AZP improved ( P < 0.05) the development of pronuclear stage embryos with a slit zona pellucida to morula and blastocyst stages as compared with ZS embryos. It is concluded that AZP improves the in vitro development of pronuclear stage embryos with intact or completely removed zona pellucida as well as micromanipulated embryos to the blastocyst stage.
ISSN:0378-4320
1873-2232
DOI:10.1016/S0378-4320(97)00048-1