A simple and sensitive bioassay for the detection of human interleukin-18/interferon- γ-inducing factor using human myelomonocytic KG-1 cells

Interleukin-18 (IL-18)/interferon- γ-inducing factor (IGIF) is a novel cytokine which plays an important role in Th1 responses. Here we describe a simple, sensitive bioassay for human IL-18 using the human myelomonocytic cell line, KG-1, which produces IFN- γ in response to human IL-18. IFN- γ produ...

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Bibliographic Details
Published in:Journal of immunological methods 1997-12, Vol.209 (2), p.187-191
Main Authors: Konishi, Kaori, Tanabe, Fujimi, Taniguchi, Mutsuko, Yamauchi, Hiroshi, Tanimoto, Tadao, Ikeda, Masao, Orita, Kunzo, Kurimoto, Masashi
Format: Article
Language:English
Subjects:
Animals
Bioassay
Biological Assay
Cross Reactions
Cytokine
Cytokines - biosynthesis
Cytokines - pharmacology
Humans
IL-18
Interferon Inducers - analysis
Interferon Inducers - pharmacology
Interferon-gamma - biosynthesis
Interleukin-18
Mice
Quantification
Sensitivity and Specificity
Tumor Cells, Cultured
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Summary:Interleukin-18 (IL-18)/interferon- γ-inducing factor (IGIF) is a novel cytokine which plays an important role in Th1 responses. Here we describe a simple, sensitive bioassay for human IL-18 using the human myelomonocytic cell line, KG-1, which produces IFN- γ in response to human IL-18. IFN- γ production induced by human IL-18 was completely blocked by an antibody against human IL-18. Human IL-18 could be measured in a concentration range from approximately 100 to 10,000 pg/ml, and intra- and inter-assay coefficient variations were both below 15%. It was possible to measure human IL-18 in human serum, cell lysate or culture supernatant by this bioassay. Thus, the human IL-18 bioassay can be expected to be useful in the investigation of the relationship between human IL-18 and various diseases or in analyzing the mechanisms of human IL-18 secretion from IL-18 producing cells.
ISSN:0022-1759
1872-7905
DOI:10.1016/S0022-1759(97)00164-6