Sequence analysis of the groESL-cotA region of the Bacillus subtilis genome, containing the restriction/modification system genes

We have determined a 35-kb sequence of the groESL-gutR-cotA (45 degrees-52 degrees) region of the Bacillus subtilis genome. In addition to the groESL, gutRB and cotA genes reported previously, we have newly identified 24 ORFs including gutA and fruC genes, encoding glucitol permease and fructokinase...

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Bibliographic Details
Published in:DNA research 1997, Vol.4 (5), p.335-339
Main Authors: Kasahara, Y, Nakai, S, Ogasawara, N, Yata, K, Sadaie, Y
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Bacillus subtilis - chemistry
Bacillus subtilis - genetics
Base Sequence
DNA Restriction-Modification Enzymes - chemistry
DNA Restriction-Modification Enzymes - genetics
DNA, Bacterial - chemistry
Electrophoresis, Agar Gel
Genome, Bacterial
Molecular Sequence Data
Open Reading Frames
Polymerase Chain Reaction
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Sorbitol - metabolism
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Summary:We have determined a 35-kb sequence of the groESL-gutR-cotA (45 degrees-52 degrees) region of the Bacillus subtilis genome. In addition to the groESL, gutRB and cotA genes reported previously, we have newly identified 24 ORFs including gutA and fruC genes, encoding glucitol permease and fructokinase, respectively. The inherent restriction/modification system genes, hsdMR and hsdMM, were mapped between groESL and gutRB, and we have identified two open reading frames (ORFs) encoding 5-methylcytosine forming DNA methyl transferase and an operon probably encoding a restriction enzyme complex. The unusual genome structure of few ORFs and lower GC content around the restriction/modification genes strongly suggests that the region originated from a bacteriophage integrated during evolution.
ISSN:1340-2838
DOI:10.1093/dnares/4.5.335