Rapid single-tube immunocapture RT-PCR for the detection of two yam potyviruses

An immunocapture reverse transcription-polymerase chain reaction (IC-RT-PCR) based assay has been developed for the detection of yam-infecting potyviruses. Based upon the same format two distinct simple tests have been developed, which allow the reliable diagnosis of yam mosaic virus and the tentati...

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Bibliographic Details
Published in:Journal of virological methods 1997-12, Vol.69 (1), p.73-79
Main Authors: Mumford, R.A, Seal, S.E
Format: Article
Language:English
Subjects:
Biological and medical sciences
Dioscorea viruses
Fundamental and applied biological sciences. Psychology
Generalities. Techniques. Transmission, epidemiology, ecology. Antiviral substances, control
Microbiology
Phytopathology. Animal pests. Plant and forest protection
Plant Diseases - virology
Plant viral diagnostics
Plant viruses and viroids
Polymerase Chain Reaction
Potyviridae
Potyvirus - genetics
Potyvirus - immunology
Potyvirus - isolation & purification
Reproducibility of Results
Reverse transcription
Sensitivity and Specificity
Solanaceae - virology
Techniques used in virology
Transcription, Genetic
Virology
Yam mild mosaic virus
Yam mosaic virus
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Summary:An immunocapture reverse transcription-polymerase chain reaction (IC-RT-PCR) based assay has been developed for the detection of yam-infecting potyviruses. Based upon the same format two distinct simple tests have been developed, which allow the reliable diagnosis of yam mosaic virus and the tentatively named yam mild mosaic virus. By using immunocapture and a single-buffer RT-PCR reaction, the test can be performed in a single tube. The tests described have been shown to exhibit a thousand-fold increase in detection sensitivity compared to existing ELISA assays.
ISSN:0166-0934
1879-0984
DOI:10.1016/S0166-0934(97)00141-9