Lack of translation of normal 7B2 mRNA levels in hypothalamic mutant vasopressin cells of the homozygous Brattleboro rat

The homozygous Brattleboro rat (di/di) synthesizes a vasopressin (VP) precursor with an aberrant C-terminus, which causes a hypothalamic form of diabetes insipidus. The neuroendocrine polypeptide 7B2 is present in VP and oxytocin (OT) neurons of the supraoptic and paraventricular nucleus of the hypo...

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Bibliographic Details
Published in:Neuroscience letters 1997-12, Vol.239 (1), p.5-8
Main Authors: Gabreëls, B.A.Th.F, Verwer, R.W.H, Sonnemans, M.A.F, Sluiter, A.A, Ang, C.W, van Leeuwen, F.W
Format: Article
Language:English
Subjects:
Animals
Associated diseases and complications
Biological and medical sciences
Compartmentalization
Diabetes. Impaired glucose tolerance
Endocrine pancreas. Apud cells (diseases)
Endocrinopathies
Endoplasmic reticulum
Female
Homozygote
Hypothalamus - cytology
Hypothalamus - metabolism
In Situ Hybridization
Male
Medical sciences
Mutation
Nerve Tissue Proteins - genetics
Neuroendocrine Secretory Protein 7B2
Neurons - metabolism
Neurosecretory Systems
Oxytocin
Pituitary Hormones - genetics
Protein Biosynthesis
Rats
Rats, Brattleboro
Reference Values
RNA, Messenger - biosynthesis
Translocon
Vasopressins - metabolism
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Summary:The homozygous Brattleboro rat (di/di) synthesizes a vasopressin (VP) precursor with an aberrant C-terminus, which causes a hypothalamic form of diabetes insipidus. The neuroendocrine polypeptide 7B2 is present in VP and oxytocin (OT) neurons of the supraoptic and paraventricular nucleus of the hypothalamus in wild type rats. However, in the di/di rat 7B2 immunoreactivity is absent in the VP cell population, whereas 7B2 levels within the OT cells are unaffected. Remarkably, there is no obvious difference in 7B2 transcript levels between VP and OT neurons in the di/di rat hypothalamus. This study shows that the presence of mRNA does not automatically result in the subsequent synthesis of its protein. Cellular mechanisms underlying this discrepancy are discussed.
ISSN:0304-3940
1872-7972
DOI:10.1016/S0304-3940(97)00883-5