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Lack of translation of normal 7B2 mRNA levels in hypothalamic mutant vasopressin cells of the homozygous Brattleboro rat
The homozygous Brattleboro rat (di/di) synthesizes a vasopressin (VP) precursor with an aberrant C-terminus, which causes a hypothalamic form of diabetes insipidus. The neuroendocrine polypeptide 7B2 is present in VP and oxytocin (OT) neurons of the supraoptic and paraventricular nucleus of the hypo...
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Published in: | Neuroscience letters 1997-12, Vol.239 (1), p.5-8 |
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container_title | Neuroscience letters |
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creator | Gabreëls, B.A.Th.F Verwer, R.W.H Sonnemans, M.A.F Sluiter, A.A Ang, C.W van Leeuwen, F.W |
description | The homozygous Brattleboro rat (di/di) synthesizes a vasopressin (VP) precursor with an aberrant C-terminus, which causes a hypothalamic form of diabetes insipidus. The neuroendocrine polypeptide 7B2 is present in VP and oxytocin (OT) neurons of the supraoptic and paraventricular nucleus of the hypothalamus in wild type rats. However, in the di/di rat 7B2 immunoreactivity is absent in the VP cell population, whereas 7B2 levels within the OT cells are unaffected. Remarkably, there is no obvious difference in 7B2 transcript levels between VP and OT neurons in the di/di rat hypothalamus. This study shows that the presence of mRNA does not automatically result in the subsequent synthesis of its protein. Cellular mechanisms underlying this discrepancy are discussed. |
doi_str_mv | 10.1016/S0304-3940(97)00883-5 |
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The neuroendocrine polypeptide 7B2 is present in VP and oxytocin (OT) neurons of the supraoptic and paraventricular nucleus of the hypothalamus in wild type rats. However, in the di/di rat 7B2 immunoreactivity is absent in the VP cell population, whereas 7B2 levels within the OT cells are unaffected. Remarkably, there is no obvious difference in 7B2 transcript levels between VP and OT neurons in the di/di rat hypothalamus. This study shows that the presence of mRNA does not automatically result in the subsequent synthesis of its protein. Cellular mechanisms underlying this discrepancy are discussed.</description><identifier>ISSN: 0304-3940</identifier><identifier>EISSN: 1872-7972</identifier><identifier>DOI: 10.1016/S0304-3940(97)00883-5</identifier><identifier>PMID: 9547170</identifier><identifier>CODEN: NELED5</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Animals ; Associated diseases and complications ; Biological and medical sciences ; Compartmentalization ; Diabetes. Impaired glucose tolerance ; Endocrine pancreas. Apud cells (diseases) ; Endocrinopathies ; Endoplasmic reticulum ; Female ; Homozygote ; Hypothalamus - cytology ; Hypothalamus - metabolism ; In Situ Hybridization ; Male ; Medical sciences ; Mutation ; Nerve Tissue Proteins - genetics ; Neuroendocrine Secretory Protein 7B2 ; Neurons - metabolism ; Neurosecretory Systems ; Oxytocin ; Pituitary Hormones - genetics ; Protein Biosynthesis ; Rats ; Rats, Brattleboro ; Reference Values ; RNA, Messenger - biosynthesis ; Translocon ; Vasopressins - metabolism</subject><ispartof>Neuroscience letters, 1997-12, Vol.239 (1), p.5-8</ispartof><rights>1997 Elsevier Science Ireland Ltd</rights><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c420t-9927b1ef61e128056b1289a26176a4ade89ab587dd27212f8ed48d51b2f332493</citedby><cites>FETCH-LOGICAL-c420t-9927b1ef61e128056b1289a26176a4ade89ab587dd27212f8ed48d51b2f332493</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2155908$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9547170$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gabreëls, B.A.Th.F</creatorcontrib><creatorcontrib>Verwer, R.W.H</creatorcontrib><creatorcontrib>Sonnemans, M.A.F</creatorcontrib><creatorcontrib>Sluiter, A.A</creatorcontrib><creatorcontrib>Ang, C.W</creatorcontrib><creatorcontrib>van Leeuwen, F.W</creatorcontrib><title>Lack of translation of normal 7B2 mRNA levels in hypothalamic mutant vasopressin cells of the homozygous Brattleboro rat</title><title>Neuroscience letters</title><addtitle>Neurosci Lett</addtitle><description>The homozygous Brattleboro rat (di/di) synthesizes a vasopressin (VP) precursor with an aberrant C-terminus, which causes a hypothalamic form of diabetes insipidus. The neuroendocrine polypeptide 7B2 is present in VP and oxytocin (OT) neurons of the supraoptic and paraventricular nucleus of the hypothalamus in wild type rats. However, in the di/di rat 7B2 immunoreactivity is absent in the VP cell population, whereas 7B2 levels within the OT cells are unaffected. Remarkably, there is no obvious difference in 7B2 transcript levels between VP and OT neurons in the di/di rat hypothalamus. This study shows that the presence of mRNA does not automatically result in the subsequent synthesis of its protein. Cellular mechanisms underlying this discrepancy are discussed.</description><subject>Animals</subject><subject>Associated diseases and complications</subject><subject>Biological and medical sciences</subject><subject>Compartmentalization</subject><subject>Diabetes. Impaired glucose tolerance</subject><subject>Endocrine pancreas. Apud cells (diseases)</subject><subject>Endocrinopathies</subject><subject>Endoplasmic reticulum</subject><subject>Female</subject><subject>Homozygote</subject><subject>Hypothalamus - cytology</subject><subject>Hypothalamus - metabolism</subject><subject>In Situ Hybridization</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Mutation</subject><subject>Nerve Tissue Proteins - genetics</subject><subject>Neuroendocrine Secretory Protein 7B2</subject><subject>Neurons - metabolism</subject><subject>Neurosecretory Systems</subject><subject>Oxytocin</subject><subject>Pituitary Hormones - genetics</subject><subject>Protein Biosynthesis</subject><subject>Rats</subject><subject>Rats, Brattleboro</subject><subject>Reference Values</subject><subject>RNA, Messenger - biosynthesis</subject><subject>Translocon</subject><subject>Vasopressins - metabolism</subject><issn>0304-3940</issn><issn>1872-7972</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><recordid>eNqFkU1v3CAQhlHUKN2m-QmROFRVe3ADGIw5VUnUL2mVSP04I4zHXVpstsCusvn1xdnVXnMaRu8zM694Ebqk5AMltLn6QWrCq1px8k7J94S0bV2JE7SgrWSVVJK9QIsj8hK9SukPIURQwc_QmRJcUkkW6GFp7F8cBpyjmZI32YVpbqcQR-OxvGF4_H53jT1swSfsJrzarUNeGW9GZ_G4yWbKeGtSWEdIqegWfAHnjSvAqzCGx93vsEn4JpqcPXQhBlyer9HpYHyCi0M9R78-f_p5-7Va3n_5dnu9rCxnJFdKMdlRGBoKlLVENF0pyrCGysZw00NpOtHKvmeSUTa00PO2F7RjQ10zrupz9Ha_dx3Dvw2krEeXZo9mgmJLSyUaQVjzLEgbwVnNZAHFHrQxpBRh0OvoRhN3mhI9R6OfotHzv2sl9VM0WpS5y8OBTTdCf5w6ZFH0NwfdJGv8UAKxLh0xRoVQpC3Yxz1W8oCtg6iTdTBZ6F0Em3Uf3DNG_gNecqra</recordid><startdate>19971212</startdate><enddate>19971212</enddate><creator>Gabreëls, B.A.Th.F</creator><creator>Verwer, R.W.H</creator><creator>Sonnemans, M.A.F</creator><creator>Sluiter, A.A</creator><creator>Ang, C.W</creator><creator>van Leeuwen, F.W</creator><general>Elsevier Ireland Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>19971212</creationdate><title>Lack of translation of normal 7B2 mRNA levels in hypothalamic mutant vasopressin cells of the homozygous Brattleboro rat</title><author>Gabreëls, B.A.Th.F ; Verwer, R.W.H ; Sonnemans, M.A.F ; Sluiter, A.A ; Ang, C.W ; van Leeuwen, F.W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c420t-9927b1ef61e128056b1289a26176a4ade89ab587dd27212f8ed48d51b2f332493</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>Associated diseases and complications</topic><topic>Biological and medical sciences</topic><topic>Compartmentalization</topic><topic>Diabetes. Impaired glucose tolerance</topic><topic>Endocrine pancreas. Apud cells (diseases)</topic><topic>Endocrinopathies</topic><topic>Endoplasmic reticulum</topic><topic>Female</topic><topic>Homozygote</topic><topic>Hypothalamus - cytology</topic><topic>Hypothalamus - metabolism</topic><topic>In Situ Hybridization</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Mutation</topic><topic>Nerve Tissue Proteins - genetics</topic><topic>Neuroendocrine Secretory Protein 7B2</topic><topic>Neurons - metabolism</topic><topic>Neurosecretory Systems</topic><topic>Oxytocin</topic><topic>Pituitary Hormones - genetics</topic><topic>Protein Biosynthesis</topic><topic>Rats</topic><topic>Rats, Brattleboro</topic><topic>Reference Values</topic><topic>RNA, Messenger - biosynthesis</topic><topic>Translocon</topic><topic>Vasopressins - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gabreëls, B.A.Th.F</creatorcontrib><creatorcontrib>Verwer, R.W.H</creatorcontrib><creatorcontrib>Sonnemans, M.A.F</creatorcontrib><creatorcontrib>Sluiter, A.A</creatorcontrib><creatorcontrib>Ang, C.W</creatorcontrib><creatorcontrib>van Leeuwen, F.W</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Neuroscience letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gabreëls, B.A.Th.F</au><au>Verwer, R.W.H</au><au>Sonnemans, M.A.F</au><au>Sluiter, A.A</au><au>Ang, C.W</au><au>van Leeuwen, F.W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Lack of translation of normal 7B2 mRNA levels in hypothalamic mutant vasopressin cells of the homozygous Brattleboro rat</atitle><jtitle>Neuroscience letters</jtitle><addtitle>Neurosci Lett</addtitle><date>1997-12-12</date><risdate>1997</risdate><volume>239</volume><issue>1</issue><spage>5</spage><epage>8</epage><pages>5-8</pages><issn>0304-3940</issn><eissn>1872-7972</eissn><coden>NELED5</coden><abstract>The homozygous Brattleboro rat (di/di) synthesizes a vasopressin (VP) precursor with an aberrant C-terminus, which causes a hypothalamic form of diabetes insipidus. The neuroendocrine polypeptide 7B2 is present in VP and oxytocin (OT) neurons of the supraoptic and paraventricular nucleus of the hypothalamus in wild type rats. However, in the di/di rat 7B2 immunoreactivity is absent in the VP cell population, whereas 7B2 levels within the OT cells are unaffected. Remarkably, there is no obvious difference in 7B2 transcript levels between VP and OT neurons in the di/di rat hypothalamus. This study shows that the presence of mRNA does not automatically result in the subsequent synthesis of its protein. Cellular mechanisms underlying this discrepancy are discussed.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><pmid>9547170</pmid><doi>10.1016/S0304-3940(97)00883-5</doi><tpages>4</tpages></addata></record> |
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subjects | Animals Associated diseases and complications Biological and medical sciences Compartmentalization Diabetes. Impaired glucose tolerance Endocrine pancreas. Apud cells (diseases) Endocrinopathies Endoplasmic reticulum Female Homozygote Hypothalamus - cytology Hypothalamus - metabolism In Situ Hybridization Male Medical sciences Mutation Nerve Tissue Proteins - genetics Neuroendocrine Secretory Protein 7B2 Neurons - metabolism Neurosecretory Systems Oxytocin Pituitary Hormones - genetics Protein Biosynthesis Rats Rats, Brattleboro Reference Values RNA, Messenger - biosynthesis Translocon Vasopressins - metabolism |
title | Lack of translation of normal 7B2 mRNA levels in hypothalamic mutant vasopressin cells of the homozygous Brattleboro rat |
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