Headspace gas chromatography with capillary column for urine alcohol determination

A headspace gas chromatographic method using a fused-silica capillary column Poraplot Q has been developed and validated for the detection and quantification of ethanol in urine. Under optimized conditions, ethanol was properly separated from acetaldehyde, acetone, isopropanol, methanol and n-propan...

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Bibliographic Details
Published in:Journal of chromatography. B, Biomedical sciences and applications Biomedical sciences and applications, 1997-12, Vol.704 (1), p.365-368
Main Authors: Corrêa, Cristiana Leslie, Pedroso, Rosemary Custódio
Format: Article
Language:English
Subjects:
Adult
Alcoholism and acute alcohol poisoning
Biological and medical sciences
Chromatography, Gas - methods
Ethanol
Ethanol - urine
Humans
Kinetics
Male
Medical sciences
Quality Control
Sensitivity and Specificity
Toxicology
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Summary:A headspace gas chromatographic method using a fused-silica capillary column Poraplot Q has been developed and validated for the detection and quantification of ethanol in urine. Under optimized conditions, ethanol was properly separated from acetaldehyde, acetone, isopropanol, methanol and n-propanol. Limits of detection (LODs) and quantification (LOQs) were 0.008 and 0.010 g/l, respectively. The precision studies within-run and between-run, using spiked urine samples (0.08, 0.8 and 2.0 g/l) showed maximum coefficients of variation 5.9 and 6.5%, respectively. Results of ethanol recovery varied from 91.6±0.8 to 103.3±1.8% over the concentration range from 0.01 to 3.20 g/l. The method was appropriate for the detection of ethanol in urine samples. This matrix can be used for monitoring alcohol abuse in the workplace and used in alcohol rehabilitation programs.
ISSN:0378-4347
1387-2273
DOI:10.1016/S0378-4347(97)00445-3