Genetic diversity among Trypanosoma brucei rhodesiense isolates from Tanzania

We compared 19 stocks of Trypanosoma brucei rhodesiense collected in 1991 and 1994 from Tanzania with representative stocks from other foci of Rhodesian sleeping sickness in Zambia, Kenya and Uganda. Stocks were characterized by isoenzyme electrophoresis, restriction fragment length polymorphisms in...

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Bibliographic Details
Published in:Parasitology 1997-12, Vol.115 (6), p.571-579
Main Authors: KOMBA, E. K., KIBONA, S. N., AMBWENE, A. K., STEVENS, J. R., GIBSON, W. C.
Format: Article
Language:English
Subjects:
Animals
Biochemistry. Physiology. Immunology. Molecular biology
Biological and medical sciences
Electrophoresis
Electrophoresis, Gel, Pulsed-Field
Female
Fundamental and applied biological sciences. Psychology
Genes, Protozoan
Genetic Variation
human trypanosomiasis
Humans
Isoenzymes - analysis
Male
Polymorphism, Restriction Fragment Length
Protozoa
Random Amplified Polymorphic DNA Technique
sleeping sickness
Tanzania
Trypanosoma brucei rhodesiense
Trypanosoma brucei rhodesiense - chemistry
Trypanosoma brucei rhodesiense - classification
Trypanosoma brucei rhodesiense - genetics
Trypanosoma brucei rhodesiense - isolation & purification
Trypanosomiasis, African - parasitology
Tubulin - genetics
Variant Surface Glycoproteins, Trypanosoma - genetics
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Summary:We compared 19 stocks of Trypanosoma brucei rhodesiense collected in 1991 and 1994 from Tanzania with representative stocks from other foci of Rhodesian sleeping sickness in Zambia, Kenya and Uganda. Stocks were characterized by isoenzyme electrophoresis, restriction fragment length polymorphisms in variant surface glycoprotein genes and random amplification of polymorphic DNA; the banding patterns obtained were coded for numerical analysis. In addition, the Tanzanian stocks were compared by pulsed field gel electrophoresis. Overall the Tanzanian stocks formed a homogeneous group and the predominant genotype isolated in 1991 was still present in the 1994 sample, although at a reduced level. The Tanzanian stocks were distinct from representative stocks from other East African foci. This observation does not support the proposal that there are northern and southern strains of T. b. rhodesiense, but is consistent with the view that T. b. rhodesiense stocks form a mosaic of different genotypes varying from focus to focus in East Africa.
ISSN:0031-1820
1469-8161
DOI:10.1017/S0031182097001856