Simultaneous determination of the two components of picrotoxin in serum by reversed-phase high-performance liquid chromatography with application to a pharmacokinetic study in rats

A reversed-phase HPLC method is reported which allows the quantification of picrotin and picrotoxinin in serum. A linear response was obtained for both drugs in the range 0.2–20 μg ml −1. The within-day and between-day precisions were 0.8–3.7% and 1.3–4.9%, respectively. The mean recoveries were gre...

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Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 1989, Vol.7 (3), p.369-375
Main Authors: Soto-Otero, R., Mendez-Alvarez, E., Sierra-Paredes, G., Galan-Valiente, J., Aguilar-Veiga, E., Sierra-Marcuño, G.
Format: Article
Language:English
Subjects:
Analysis
Animals
Biological and medical sciences
Chromatography, High Pressure Liquid
General pharmacology
HPLC
Injections, Intraperitoneal
Male
Medical sciences
pharmaceutical analysis
pharmacokinetics
Pharmacology. Drug treatments
picrotin
picrotoxin
Picrotoxin - analogs & derivatives
Picrotoxin - blood
Picrotoxin - pharmacokinetics
picrotoxinin
rat
Rats
Rats, Inbred Strains
Reference Standards
Reversed-phase
serum
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Summary:A reversed-phase HPLC method is reported which allows the quantification of picrotin and picrotoxinin in serum. A linear response was obtained for both drugs in the range 0.2–20 μg ml −1. The within-day and between-day precisions were 0.8–3.7% and 1.3–4.9%, respectively. The mean recoveries were greater than 94.2%. The method was applied to a pharmacokinetic study following intraperitoneal (i.p.) administration of 3 mg kg −1 of picrotoxin in rats. The obtained data suggest a relatively slow absorption after i.p. administration followed by a rapid elimination from the central compartment according to a one-compartment open model. The elimination half-lives were 0.340 ± 0.0308 h for picrotin and 0.312 ± 0.0241 h for picrotoxinin.
ISSN:0731-7085
1873-264X
DOI:10.1016/0731-7085(89)80104-9