The effect of an intravenous infusion of IGF-I and insulin on IGFBP-1, IGFBP-3, acid labile subunit, free and bound IGF-I, catecholamines and potassium in normal volunteers during an amino acid and glucose clamp

• Published in: Clinical endocrinology (Oxford) 1997-12, Vol.47 (6), p.685-691
• Main Authors: Russell-Jones, D. L., Umpleby, A. M., Shojaee-Moradie, F., Boroujerdi, M. A., Jones, R. H., Baxter, R. C., Sönksen, P. H.
• Format: Article
• Language: English
• Subjects: Adult; Amino Acids - metabolism; Biological and medical sciences; Carrier Proteins - blood; Catecholamines - blood; Dopamine - blood; Epinephrine - blood; Functional investigation of endocrine glands and genital system; Glucose Clamp Technique; Glycoproteins - blood; Humans; Infusions, Intravenous; Insulin - analysis; Insulin - pharmacology; Insulin-Like Growth Factor Binding Protein 1 - blood; Insulin-Like Growth Factor Binding Protein 3 - blood; Insulin-Like Growth Factor Binding Proteins - blood; Insulin-Like Growth Factor I - analysis; Insulin-Like Growth Factor I - pharmacology; Investigative techniques, diagnostic techniques (general aspects); Male; Medical sciences; Middle Aged; Potassium - blood
• ISSN: 0300-0664; 1365-2265
• DOI: 10.1046/j.1365-2265.1997.3161133.x

OBJECTIVES To investigate the effects of IGF‐I and insulin at doses equipotent with respect to hypoglycaemic effect on IGF‐I concentrations (free and bound), IGF binding proteins, catecholamines and potassium levels. DESIGN A glucose and amino acid clamp technique was used to investigate the effects of a 3 h intravenous infusion of either IGF‐I 43.7 pmol/kg/min (20 μg/kg/ h) or insulin 3.4 pmol/kg/min (0.5 mU/kg/min). MEASUREMENTS Circulating levels of total IGF‐I, free and bound IGF‐I, insulin, IGFBP‐1, IGFBP‐3, ALS, catecholamines and potassium were measured. PATIENTS 6 normal human volunteers aged 21–49. RESULTS During the IGF‐I infusion, IGF‐I levels increased (P