Determination of encainide and its metabolites by high-performance liquid chromatography

Encainide (ENC) and its metabolites O-demethylencainide (ODE), 3-methoxy- O-demethylencainide (MODE), N-demethylencainide (NDE) and bis- N,O-demethylencainide (NODE) have been measured by two HPLC procedures. The method of Mayol using a μPorasil column with ethanol—water—methanesulphonic acid as mob...

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Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 1989, Vol.7 (3), p.355-359
Main Authors: Selinger, K., Crawhall, J.C.
Format: Article
Language:English
Subjects:
Analysis
Anilides - analysis
Anilides - blood
Anilides - metabolism
Anti-Arrhythmia Agents - analysis
Anti-Arrhythmia Agents - blood
Anti-Arrhythmia Agents - metabolism
Biological and medical sciences
Biotransformation
Chromatography, High Pressure Liquid
Dealkylation
Encainide
Encainide metabolites
General pharmacology
HPLC
Humans
Medical sciences
metabolic demethylation
Pharmacology. Drug treatments
Spectrophotometry, Ultraviolet
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Summary:Encainide (ENC) and its metabolites O-demethylencainide (ODE), 3-methoxy- O-demethylencainide (MODE), N-demethylencainide (NDE) and bis- N,O-demethylencainide (NODE) have been measured by two HPLC procedures. The method of Mayol using a μPorasil column with ethanol—water—methanesulphonic acid as mobile phase was not able to separately measure NODE and NDE in plasma. A new method is described using a μBondapak Phenyl column with acetonitrile—phosphate buffer (0.05 M, pH 7.5) that yields satisfactory separation of ENC and its metabolites. NODE was not identified as a metabolite in 23 patients analysed.
ISSN:0731-7085
1873-264X
DOI:10.1016/0731-7085(89)80102-5