Control and manipulation of gene expression during tomato fruit ripening

Ripening is a complex developmental process involving changes in the biochemistry, physiology and gene expression of the fruit. It is an active process characterised by changes in all cellular compartments. cDNA cloning has been used as an approach to analyse changes in gene expression during fruit...

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Bibliographic Details
Published in:Plant molecular biology 1989-09, Vol.13 (3), p.303-311
Main Authors: Schuch, W. (ICI Seeds, Bracknell, Berks. (UK). Plant Biotechnology Section), Bird, C.R, Ray, J, Smith, C.J.S, Watson, C.F, Morris, P.C, Gray, J.E, Arnold, C, Seymour, G.B, Tucker, G.A, Grierson, D
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Biological and medical sciences
Biotechnology
Cloning, Molecular
DNA - genetics
Eukaryotes
Fruit - genetics
Fruit - growth & development
Fundamental and applied biological sciences. Psychology
Gene Expression
GENETIC ENGINEERING
Genetic engineering applications
Genetic technics
Genetics and breeding of economic plants
GENIE GENETIQUE
INGENIERIA GENETICA
LYCOPERSICON ESCULENTUM
MADURACION
MATURATION
Methods. Procedures. Technologies
Mutant screening
Mutation
Plant breeding: fundamental aspects and methodology
Plant Development
Plants - genetics
Polygalacturonase - genetics
RNA, Antisense - genetics
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Summary:Ripening is a complex developmental process involving changes in the biochemistry, physiology and gene expression of the fruit. It is an active process characterised by changes in all cellular compartments. cDNA cloning has been used as an approach to analyse changes in gene expression during fruit ripening. This has revealed that several genes are switched on specifically during fruit ripening, including one encoding polygalacturonase (PG), a major cell wall protein. These cDNA clones have been used to study the expression of the genes in normal and ripening mutant fruits, and under environmental stress conditions. The PG gene has been isolated and it has been demonstrated that 1450 bases 5' of the coding region are sufficient for the tissue- and development-specific expression of a bacterial marker gene in transgenic tomatoes. Antisense RNA techniques have been developed to generate novel mutant tomatoes in which the biochemical function of this enzyme and its involvement in fruit softening has been tested.
ISSN:0167-4412
1573-5028
DOI:10.1007/BF00025318