Occurrence of 13( S)-Hydroxyoctadecadienoic Acid in Biological Samples

The oxygenated metabolite of linoleic acid, 13( S)-hydroxyoctadecadienoic acid has recently been shown to play a role in cellular regulation. To detect this molecule in biological systems, we recently developed a specific polyclonal antibody. Using this antibody, we report the presence of 13( S)-hyd...

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Bibliographic Details
Published in:Prostaglandins 1997-12, Vol.54 (6), p.875-880
Main Authors: Spindler, S.A, Clark, K.S, Blackburn, M.L, Bull, A.W, Reddy, R.G
Format: Article
Language:English
Subjects:
13-Hydroxyocadecadienoic acid
Animals
dietary fatty acids
Gas Chromatography-Mass Spectrometry
Goats
Humans
immunoassay
Immunoenzyme Techniques
linoleic acid
Linoleic Acids - analysis
Linoleic Acids - blood
Linoleic Acids - urine
oxidation
Sensitivity and Specificity
Stereoisomerism
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Summary:The oxygenated metabolite of linoleic acid, 13( S)-hydroxyoctadecadienoic acid has recently been shown to play a role in cellular regulation. To detect this molecule in biological systems, we recently developed a specific polyclonal antibody. Using this antibody, we report the presence of 13( S)-hydroxyoctadecadienoic acid in human urine, cell culture media, and untreated goat serum for the first time by a specific, sensitive, and rapid enzyme immunoassay. Furthermore, the enzyme linked immunosorbent assay data are verified by gas chromatography/mass spectrometry analysis of the same samples.
ISSN:0090-6980
DOI:10.1016/S0090-6980(97)00185-8