Prenylated Proteins: The Structure of the Isoprenoid Group

The mevalonate-derived portion of a prenylated protein from Chinese hamster ovary cells has been established as diterpenoid (C$_{20}$). This group is linked to a carboxyl-terminal cysteine as a thioether. It was removed from the protein by hydrazinolysis followed by Raney nickel desulfurization, and...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1990-01, Vol.247 (4940), p.318-320
Main Authors: Rilling, H. C., Breunger, E., Epstein, W. W., Crain, P. F.
Format: Article
Language:English
Subjects:
Analysis
Animals
Biochemistry
Biological and medical sciences
Cell Line
Chemistry
CHO cells
Chromatography
Cricetinae
Cysteine proteinases
Desulfuration
Desulfurization
Diterpenes
Diterpenes - metabolism
Female
Fundamental and applied biological sciences. Psychology
Gas chromatography
Gas Chromatography-Mass Spectrometry
Hamsters
Hydrocarbons
Laboratory rodents
Mass spectra
Mass spectrometry
Mevalonic Acid - metabolism
Molecular and cellular biology
Molecular genetics
Molecular Structure
Ovary
Protein Precursors - metabolism
Protein Processing, Post-Translational
Protein research
Proteins
Proteins - metabolism
Rodents as laboratory animals
Solvents
Sulfides
Terpenoids
Translation. Translation factors. Protein processing
Yeasts
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Summary:The mevalonate-derived portion of a prenylated protein from Chinese hamster ovary cells has been established as diterpenoid (C$_{20}$). This group is linked to a carboxyl-terminal cysteine as a thioether. It was removed from the protein by hydrazinolysis followed by Raney nickel desulfurization, and the resulting hydrocarbon fraction was analyzed by gas chromatography-mass spectrometry.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.2296720