Sonodynamically-induced in vitro cell damage enhanced by adriamycin

• Published in: Cancer letters 1997-12, Vol.121 (2), p.195-201
• Main Authors: Umemura, Shin-ichiro, Yumita, Nagahiko, Okano, Yukihiro, Kaneuchi, Masahiro, Magario, Naoki, Ishizaki, Midori, Shimizu, Keiko, Sano, Yasuko, Umemura, Koshiro, Nishigaki, Ryuichiro
• Format: Article
• Language: English
• Subjects: Active oxygen; Adriamycin; Animals; Antibiotics, Antineoplastic - therapeutic use; Antineoplastic agents; Biological and medical sciences; Cell damage; Cell Survival - physiology; Combined Modality Therapy; Combined treatments (chemotherapy of immunotherapy associated with an other treatment); Doxorubicin - therapeutic use; Electron Spin Resonance Spectroscopy; Free Radical Scavengers - pharmacology; Linear Models; Male; Medical sciences; Mice; Mice, Inbred ICR; Nitrogen Oxides - metabolism; Oxygen - metabolism; Pharmacology. Drug treatments; Reactive Oxygen Species - metabolism; Sarcoma 180 - metabolism; Sarcoma 180 - pathology; Sarcoma 180 - therapy; Sonochemistry; Tumor Cells, Cultured; Ultrasonic Therapy - methods; Ultrasound
• ISSN: 0304-3835; 1872-7980
• DOI: 10.1016/S0304-3835(97)00354-6

Sonodynamically-induced cell damage and active oxygen generation enhanced by adriamycin (ADM) were compared in the same in vitro insonation set-up. Significant enhancement of the rates of both ultrasonically-induced cell damage and nitroxide generation was demonstrated with 40–160 μM ADM. Both rates correlated very well resulting in a correlation coefficient of more than 0.99. The enhancement of both rates was suppressed by 10 mM histidine. These results are consistent with the hypothesis that ultrasonically-generated active oxygen plays a major role in the sonodynamically-induced cell damage enhanced by ADM.