Chromosome engineering in Saccharomyces cerevisiae by using a site-specific recombination system of a yeast plasmid

We have developed an effective method to delete or invert a chromosomal segment and to create reciprocal recombination between two nonhomologous chromosomes in Saccharomyces cerevisiae, using the site-specific recombination system of pSR1, a circular cryptic DNA plasmid resembling 2 micrometers DNA...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Bacteriology 1990-02, Vol.172 (2), p.610-618
Main Authors: Matsuzaki, H. (Osaka University, Osaka, Japan), Nakajima, R, Nishiyama, J, Araki, H, Oshima, Y
Format: Article
Language:English
Subjects:
Base Sequence
Biological and medical sciences
CHROMOSOME
Chromosome Deletion
Chromosome Inversion
Chromosomes, Fungal
CROMOSOMAS
DNA
Fundamental and applied biological sciences. Psychology
genes
Genetic Engineering - methods
Genic rearrangement. Recombination. Transposable element
GENIE GENETIQUE
Haploidy
INGENIERIA GENETICA
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Oligonucleotide Probes
PLASMIDE
PLASMIDIOS
Plasmids
RECOMBINACION
RECOMBINAISON
Recombination, Genetic
Restriction Mapping
SACCHAROMYCES CEREVISIAE
Saccharomyces cerevisiae - genetics
transformation
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We have developed an effective method to delete or invert a chromosomal segment and to create reciprocal recombination between two nonhomologous chromosomes in Saccharomyces cerevisiae, using the site-specific recombination system of pSR1, a circular cryptic DNA plasmid resembling 2 micrometers DNA of S. cerevisiae but originating from another yeast, Zygosaccharomyces rouxii. A 2.1-kilobase-pair DNA fragment bearing the specific recombination site on the inverted repeats of pSR1 was inserted at target sites on a single or two different chromosomes of S. cerevisiae by using integrative vectors. The cells were then transformed with a plasmid bearing the R gene of pSR1, which encodes the site-specific recombination enzyme and is placed downstream of the GAL1 promoter. When the transformants were cultivated in galactose medium, the recombination enzyme produced by expression of the R gene created the modified chromosome(s) by recombination between two specific recombination sites inserted on the chromosome(s)
ISSN:0021-9193
1098-5530
1067-8832
DOI:10.1128/jb.172.2.610-618.1990