Altered vascular function in early stages of heart failure in hamsters

• Published in: Journal of cardiac failure 1997-12, Vol.3 (4), p.311-318
• Main Authors: Crespo, Maria J., Altieri, Pablo I., Escobales, Nelson
• Format: Article
• Language: English
• Subjects: acetylcholine; angiotensin II; angiotensin-converting enzyme; Animals; Aorta - physiology; Cricetinae; Dose-Response Relationship, Drug; endothelial dysfunction; Endothelium, Vascular - drug effects; Endothelium, Vascular - physiology; Heart Failure - physiopathology; indomethacin; Indomethacin - pharmacology; Male; Mesocricetus; Norepinephrine - pharmacology; Renin-Angiotensin System - physiology; Syrian hamsters; vascular tone; Vasoconstriction - drug effects; Vasoconstrictor Agents - pharmacology
• ISSN: 1071-9164; 1532-8414
• DOI: 10.1016/S1071-9164(97)90031-2

Background: Congestive heart failure is a clinical condition associated with alterations in the normal balance of neurohumoral agents and factors acting on the vascular wall. The etiology of this condition, however, remains largely undefined. To help elucidate the pathophysiology of this disease, vascular function and angiotensin-converting enzyme activity were evaluated in 2-month-old Syrian cardiomyopathic hamsters (SCHs) that had not yet developed heart failure. Age-matched normal hamsters were used as control hamsters. Methods and Results: Vascular function studies included determinations of contractile responses of aortic rings to 0.1 μM angiotensin II and 0.1 μM norepinephrine. In addition, endothelial function was evaluated by the vasorelaxant action of acetylcholine on norepinephrine-precontracted aortic rings. The results indicate that the pressor effect of angiotensin II (0.1 μM) was 35% greater in aortic rings from SCHs than that observed in control animals. This effect is specific for angiotensin II because the contraction induced by NE (0.1 μM) was similar in both of these strains. Angiotensin-converting enzyme activity was threefold higher in aorta homogenates from SCHs but normal in plasma and heart tissue when compared with control hamsters. Aortic ring preparations from SCHs also exhibited endothelial dysfunction because the maximal relaxation elicited by 10 μM acetylcholine was reduced 53%. Concentration-response curves with acetylcholine yielded EC 50 values that were threefold lower in SCHs (97.2 ± 0.1 nM) than in control animals (286 ± 7 nM). Indomethacin (1 μM) increased the vasorelaxant effect of acetylcholine 28% in SCHs and shifted to the left the concentration-response curve of this agonist, suggesting an increased relaxation with the cyclooxygenase inhibitor. No effect of indomethacin on acetylcholineinduced relaxation was observed in control animals. Sodium nitroprusside induced similar relaxations in both control animals and SCHs, suggesting that the vascular smooth muscle response is normal in SCH. Conclusions: Altogether these results point to a state of enhanced vascular contractility in young SCHs that could predispose these animals to develop heart failure, the enhanced vascular contractility could result from increased activity of the local renin-angiotensin system, augmented vascular response to angiotensin II, reduced nitric oxide synthesis, and enhanced production of prostaglandins.