Diagnosis of Astrovirus Gastroenteritis by Antigen Detection with Monoclonal Antibodies

An enzyme-linked immunosorbent assay (ELISA), based on monoclonal antibodies to the astrovirus group antigen, was designed for the detection of astroviruses in stools of patients with gastroenteritis. Compared to immune electron microscopy used as the standard test, the sensitivity of the astrovirus...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of infectious diseases 1990-02, Vol.161 (2), p.226-229
Main Authors: Herrmann, John E., Nowak, Nancy A., Perron-Henry, Dorothy M., Hudson, Richard W., Cubitt, W. David, Blacklow, Neil R.
Format: Article
Language:English
Subjects:
Adenoviruses
Antibodies
Antibodies, Monoclonal - immunology
Antibodies, Viral - immunology
Antigens
Antigens, Viral - analysis
Antigens, Viral - immunology
Antiserum
Biological and medical sciences
Cross Reactions
Electron microscopy
Enzyme linked immunosorbent assay
Feces - microbiology
Gastroenteritis
Gastroenteritis - diagnosis
Gastroenteritis - microbiology
Human viral diseases
Humans
Infectious diseases
Major Articles
Mamastrovirus - immunology
Mamastrovirus - isolation & purification
Mamastrovirus - ultrastructure
Medical sciences
Microscopy, Electron
Monoclonal antibodies
Predictive Value of Tests
Rotavirus
Viral diseases
Viral diseases of the digestive system
Virus Diseases - diagnosis
Virus Diseases - microbiology
Viruses
Viruses, Unclassified - immunology
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:An enzyme-linked immunosorbent assay (ELISA), based on monoclonal antibodies to the astrovirus group antigen, was designed for the detection of astroviruses in stools of patients with gastroenteritis. Compared to immune electron microscopy used as the standard test, the sensitivity of the astrovirus ELISA was 91% (31/34)and the specificity was 96% (54/56). All five of the known astrovirus serotypes could bedetected in 16 samples on which serotyping was done. In tests on 155 stools containing other enteric viruses, including adenoviruses, rotaviruses, caliciviruses, Hawaii virus, Snow Mountain virus, and Norwalk virus (30, 20, 10, 24, 4, and 7 samples, respectively), only 3 were positive in the astrovirus ELISA. The combined specificity for all astrovirus immune electron microscopy-negative samples was 98% (206/2U). The results demonstrate that the new ELISA provides a sensitive and specific means for the diagnosis of astrovirus gastroenteritis.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/161.2.226