Blastocoele formation and cell allocation to the inner cell mass and trophectoderm in haploid and diploid pig parthenotes developing in vitro

The objective of this study was to determine developmental pattern and cell allocation to the inner cell mass and trophectoderm in haploid and diploid embryos following parthenogenetic activation. In vitro matured porcine oocytes were activated by ethanol treatment and cultured in the presence or ab...

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Bibliographic Details
Published in:Zygote (Cambridge) 1997-11, Vol.5 (4), p.365-370
Main Authors: Kim, Nam-Hyung, Uhm, Sang Jun, Ju, Jin Young, Lee, Hoon Taek, Chung, Kil Saeng
Format: Article
Language:English
Subjects:
Animals
blastocyst
Blastocyst - cytology
Cell Nucleus
Cells, Cultured
Cytochalasin B - pharmacology
Diploidy
Ethanol - pharmacology
Female
Haploidy
Inner cell mass
Oocytes
Parthenogenesis
Parthenogenesis - physiology
Pig
Ploidy
Swine
Trophectoderm
Trophoblasts - cytology
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Summary:The objective of this study was to determine developmental pattern and cell allocation to the inner cell mass and trophectoderm in haploid and diploid embryos following parthenogenetic activation. In vitro matured porcine oocytes were activated by ethanol treatment and cultured in the presence or absence of cytochalasin B for 5h. The oocytes were then cultured in the NCSU23 for 9 days. The combined treatment with cytochalasin B following ethanol treatment did not increase (p >0.1) the incidence of activation. The incidence of development to the blastocyst stage was higher (p
ISSN:0967-1994
1469-8730
DOI:10.1017/S0967199400003944