Assay of a Urinary CF-Lectin Factor as a Second Diagnostic Test for Cystic Fibrosis

Analysis of urine samples for a lectin-like factor in patients with a diagnosis of cystic fibrosis (CF) revealed that the low molecular weight (MW) factor is present in urine only from such patients and not from heterozygous carriers of the CF gene or from normal controls. The urine fraction contain...

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Bibliographic Details
Published in:The American journal of the medical sciences 1990-02, Vol.299 (2), p.103-106
Main Authors: Lieberman, Jack, Schwartzman, Morton N.
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aminoglycosides
Biological and medical sciences
Chlorides - analysis
Chromatography, Gel
Chromatography, Ion Exchange
Cystic Fibrosis
Cystic Fibrosis - diagnosis
Cystic Fibrosis - urine
Female
Humans
Investigative techniques, diagnostic techniques (general aspects)
Lectin
Lectins - urine
Male
Medical sciences
Molecular Weight
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Sweat - analysis
Sweat Test
Tobramycin - pharmacology
Urine
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Summary:Analysis of urine samples for a lectin-like factor in patients with a diagnosis of cystic fibrosis (CF) revealed that the low molecular weight (MW) factor is present in urine only from such patients and not from heterozygous carriers of the CF gene or from normal controls. The urine fraction containing the factor must be separated from whole urine by a gel filtration column (TSK-20) for the activity to appear. The assay requires addition of an aliquot of normal serum to provide the necessary IgM to which the CF-factor binds, resulting in the lectin-like activity that causes agglutination of mouse erythrocytes. All 22 CF patients tested, who were not receiving intravenous (IV) antibiotics had positive CF-lectin urinary activity, whereas five others receiving IV aminoglycosides were negative. Four patients with a clinical diagnosis of CF, but with normal sweat tests (35–54 mEq/L), all had positive urinary CF-lectin tests. A blind study in which urine samples were shipped from Miami, FL to Sepulveda, CA was completely successful in correctly identifying 11 samples from CF patients as compared with ten from non-CF patients. It was concluded that an assay for urinary CF-lectin factor is specific and reliable for confirming a diagnosis of CF when the sweat test is indeterminate, and when patients have not received recent IV aminoglycosides.
ISSN:0002-9629
1538-2990
DOI:10.1097/00000441-199002000-00005