Millimolar concentrations of ascorbic acid in purified human mononuclear leukocytes. Depletion and reaccumulation

Ascorbic acid (vitamin C) was found in isolated human mononuclear leukocytes and their purified components in millimolar concentration. Intracellular ascorbic acid was depleted greater than 96% during cell culture and was rapidly reaccumulated after addition of physiologic concentrations of ascorbic...

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Bibliographic Details
Published in:The Journal of biological chemistry 1990-02, Vol.265 (5), p.2584-2587
Main Authors: BERGSTEN, P, AMITAI, G, KEHRL, J, DHARIWAL, K. R, KLEIN, H. G, LEVINE, M
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Ascorbic Acid - blood
B-Lymphocytes - analysis
Biological and medical sciences
Blood Preservation
Cells, Cultured
Chromatography, High Pressure Liquid - methods
Coenzymes, vitamins
Fundamental and applied biological sciences. Psychology
Humans
Monocytes - analysis
Other biological molecules
T-Lymphocytes - analysis
Time Factors
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Summary:Ascorbic acid (vitamin C) was found in isolated human mononuclear leukocytes and their purified components in millimolar concentration. Intracellular ascorbic acid was depleted greater than 96% during cell culture and was rapidly reaccumulated after addition of physiologic concentrations of ascorbic acid to the extracellular medium. Purified cells maintained concentration gradients of ascorbic acid as large as 100-fold across the plasma membrane. The ability to vary intracellular ascorbic acid concentrations over such a wide range makes it possible for the first time in these cells to study ascorbic acid function in direct relationship to intracellular concentration.
ISSN:0021-9258
1083-351X
DOI:10.1016/s0021-9258(19)39841-2