Nitric Oxide-Induced Expression of C-Reactive Protein in Islet Cells as a Very Early Marker for Islet Stress in the Rat Pancreas

In searches for marker molecules specifically expressed in nitric oxide-treated islet cells as a means to recognize early events in islet destruction, we now establish the presence of neo-C-reactive protein (neoCRP) in rat islet cells as early as 2 hr after treatment. We detected this altered molecu...

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Bibliographic Details
Published in:Nitric oxide 1997-06, Vol.1 (3), p.254-262
Main Authors: Fehsel, Karin, Plewe, Dirk, Kolb-Bachofen, Victoria
Format: Article
Language:English
Subjects:
acute-phase response
Animals
Base Sequence
C-Reactive Protein - genetics
DNA Primers
Gene Expression Regulation - drug effects
heat shock
Immunohistochemistry
Islets of Langerhans - drug effects
Islets of Langerhans - metabolism
Islets of Langerhans - pathology
nitric oxide
Nitric Oxide - pharmacology
Oxidative Stress
Polymerase Chain Reaction
Rats
Rats, Wistar
RNA, Messenger - genetics
RNA, Messenger - metabolism
streptozotocin
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Summary:In searches for marker molecules specifically expressed in nitric oxide-treated islet cells as a means to recognize early events in islet destruction, we now establish the presence of neo-C-reactive protein (neoCRP) in rat islet cells as early as 2 hr after treatment. We detected this altered molecular form of the acute-phase-reactant C-reactive protein (CRP) using immunocytochemistry with an anti-neoCRP-specific monoclonal antibody as well as reverse transcription–polymerase chain reaction with CRP-specific primers andin situhybridization to demonstrate the presence of CRP-specific mRNA. After induction of a generalized inflammatory reaction in rats with heat-inactivatedCorynebacterium parvum in vivo,neoCRP expression in islets is also found and within the pancreas restricted to pancreatic islet cells only. Our findings suggest an early heat-shock-like expression of this molecule in response to local nitrite oxide production or to exogeneously added nitric oxide in islet cells.
ISSN:1089-8603
1089-8611
DOI:10.1006/niox.1997.0124