Banana bunchy top virus DNA-3 encodes the viral coat protein

Banana bunchy top virus (BBTV) has a multicomponent genome consisting of at least six circular single-stranded DNAs each with a single large open reading frame (ORF) in the virion sense. A protein of approximately 20 kDa has been associated with purified virions and is assumed to be the viral coat p...

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Bibliographic Details
Published in:Archives of virology 1997, Vol.142 (8), p.1673-1680
Main Authors: Wanitchakorn, R, Harding, R.M, Dale, J.L
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Banana bunchy top virus
Biological and medical sciences
Blotting, Western
Capsid - chemistry
Capsid Proteins
Fundamental and applied biological sciences. Psychology
genomics
Microbiology
Molecular Sequence Data
Morphology, structure, chemical composition, physicochemical properties
Phytopathology. Animal pests. Plant and forest protection
Plant Viruses - genetics
Plant Viruses - immunology
Plant viruses and viroids
Rabbits
Systematics. Structure, properties and multiplication. Genetics
Virology
Zingiberales - virology
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Summary:Banana bunchy top virus (BBTV) has a multicomponent genome consisting of at least six circular single-stranded DNAs each with a single large open reading frame (ORF) in the virion sense. A protein of approximately 20 kDa has been associated with purified virions and is assumed to be the viral coat protein. The N-terminus of this protein was sequenced and compared to the predicted amino acid sequence of the large ORF of BBTV DNA-1 to 6. This comparison indicated that the ORF of BBTV DNA-3, which potentially encodes a protein of 19.3 kDa, was the coat protein gene of BBTV. The ORF sequence of BBTV DNA-3 was cloned into a prokaryotic expression vector, pMAL-c2, and the resulting maltose binding-BBTV coat protein fusion product was purified and used for the production of polyclonal antiserum in a rabbit. The resultant antiserum was able to detect the presence of BBTV in infected leaf tissue confirming that the large virion sense ORF of BBTV DNA-3 encodes the viral coat protein.
ISSN:0304-8608
1432-8798
DOI:10.1007/s007050050188