Activity of Chinchilla laniger spermatozoa collected by electroejaculation and cryopreserved

Because reproductive studies and the application of assisted reproductive techniques are relevant issues for an endangered species such as Chinchila laniger, the availability of a source of viable spermatozoa becomes of utmost importance. In this paper, we evaluate several functional parameters (mot...

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Bibliographic Details
Published in:Theriogenology 1998-12, Vol.50 (8), p.1239-1249
Main Authors: Ponce, A.A, Carrascosa, R.E, Aires, V.A, Fiol de Cuneo, M, Ruiz, R.D, Ponzio, M.F, Lacuara, J.L
Format: Article
Language:English
Subjects:
acrosome
Acrosome - physiology
Animals
Cell Size
Cell Survival
Chinchilla - physiology
chinchillas
Cryopreservation
Ejaculation
Electric Stimulation
electroejaculation
Hypotonic Solutions
Male
motility
Semen Preservation
Sperm Motility
spermatozoa
Spermatozoa - physiology
viability
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Summary:Because reproductive studies and the application of assisted reproductive techniques are relevant issues for an endangered species such as Chinchila laniger, the availability of a source of viable spermatozoa becomes of utmost importance. In this paper, we evaluate several functional parameters (motility, viability, response to hypoosmotic swelling test and acrosomal integrity) of fresh or frozen-thawed spermatozoa. Electro-ejaculation trials (50-cyc/sec sinusoidal wave was applied for 5 of every 10 sec) were successful in all unanesthetized animals. After volume (108.3 +/- 12.0 microliter, n = 15) and concentration (421.8 +/- 34.4 X 10(6) cells/mL, n = 15) measurements, the above mentioned parameters were determined. In frozen-thawed semen samples sperm motility, viability, hypoosmotic swelling test and acrosomal integrity were significantly lower than in fresh semen samples. The results clearly indicated that electro-ejaculation is a useful method for evaluating spermatozoa for genetic analyses or for use in Al in this species. In addition, the cryopreservation procedure in this study preserved adequate levels of functional sperm activity.
ISSN:0093-691X
1879-3231
DOI:10.1016/S0093-691X(98)00223-4