The effect of oocyte size and bitch age upon oocyte nuclear maturation in vitro

In vitro maturation in the bitch has yet to be fully investigated, and perfection of the technique is essential for future gamete salvage programs in endangered canine species. For optimal success with these techniques, knowledge of the individual animal and of oocyte effects upon maturational compe...

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Bibliographic Details
Published in:Theriogenology 1998-04, Vol.49 (5), p.957-966
Main Authors: Hewitt, D.A, England, G.C.W
Format: Article
Language:English
Subjects:
AGE
AGE DIFFERENCES
Age Factors
Animals
bitch
Cattle
Cell Nucleus - physiology
Cell Nucleus - ultrastructure
Cell Separation - methods
Cell Size
Cells, Cultured
CHIEN
Conservation of Natural Resources
Culture Media
DIAMETER
DIAMETRE
DIAMETRO
DIMENSION
DIMENSIONS
DOGS
EDAD
EXPERIMENTACION IN VITRO
EXPERIMENTATION IN VITRO
Female
FEMALES
FEMELLE
gamete salvage
HEMBRA
IN VITRO EXPERIMENTATION
in vitro maturation
MADURACION
MATURATION
MEIOSE
MEIOSIS
METAPHASE
oocyte
Oocytes - cytology
Oocytes - physiology
Oogenesis - physiology
OVA
Ovarian Follicle - cytology
Ovarian Follicle - physiology
OVULE
OVULO
PERRO
Serum Albumin, Bovine
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Summary:In vitro maturation in the bitch has yet to be fully investigated, and perfection of the technique is essential for future gamete salvage programs in endangered canine species. For optimal success with these techniques, knowledge of the individual animal and of oocyte effects upon maturational competence would be useful. Two factors were therefore studied using an aceto-orcein staining technique, which has been shown to be effective for monitoring nuclear maturation of canine oocytes following oocyte culture in medium supplemented with bovine serum albumin (BSA). Oocytes of different sizes were cultured in vitro and their nuclear maturation monitored. It was shown that the selection of oocytes which had acquired meiotic competence through adequate intrafollicular growth was important for in vitro maturation. In vitro maturation of oocytes from bitches aged 1 to 6 yr, and from those 7 yr and older was then compared, and it was found that oocytes from young bitches had a greater potential to mature than those collected from the older animals.
ISSN:0093-691X
1879-3231
DOI:10.1016/S0093-691X(98)00044-2