Preliminary results of hemizona assay (HZA) as a fertility test for canine spermatozoa

The Hemizona assay (HZA) is considered to be an effective test for predicting the fertilizing potential of spermatozoa. It is a functional test that distinguishes the zona-binding capacity of spermatozoa from fertile and infertile males. The objective of this study was to validate the HZA for canine...

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Bibliographic Details
Published in:Theriogenology 1998-07, Vol.50 (2), p.195-204
Main Authors: Mayenco-Aguirre, A.M. (Universidad Complutense, Madrid, Spain.), Cortes, A.B.P
Format: Article
Language:English
Subjects:
Animals
BINDING
BIOASSAYS
CHIEN
dog
DOGS
Dogs - physiology
ENSAYO BIOLOGICO
ESPERMATOZOO
Female
FERTILIDAD
FERTILITE
FERTILITY
Fertility - physiology
fertility test
homologous hemizona
MACHO
MALE
MALES
OOCYTES
OVA
OVULE
OVULO
PERRO
Reproductive Techniques - veterinary
Sperm-Ovum Interactions - physiology
SPERMATOZOA
Spermatozoa - physiology
SPERMATOZOIDE
TEST BIOLOGIQUE
ZONA PELLUCIDA
Zona Pellucida - physiology
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Summary:The Hemizona assay (HZA) is considered to be an effective test for predicting the fertilizing potential of spermatozoa. It is a functional test that distinguishes the zona-binding capacity of spermatozoa from fertile and infertile males. The objective of this study was to validate the HZA for canine spermatozoa, as a test for diagnosing canine male fertility status. Various parameters that affect binding capacity were examined: the presence of an adequate number of capacitated and motile spermatozoa for an HZA, the influence of fertility status, sperm-binding variability within fertile dogs over 60 d, variability in sperm-binding capacity of different oocytes, the lower limit number of spermatozoa binding to a zona from the fertile control, and evaluation of HZI to determine the fertilizing capacity of spermatozoa. Hemizonae were obtained from frozen oocytes of spayed bitches. The oocytes were manually cut into nearly equal halves. Spermatozoa were capacitated by swim-up and 1 h incubation at 37°C in modified Ham's F10 medium. Spermatozoa and hemizonae were co-incubated in 100-μL drops at 37°C for 1 h. Spermatozoa from 7 fertile and 3 infertile dogs were used for this study. The optimal sperm concentration for hemizona insemination was 1x10 6/mL capacitated and motile spermatozoa. A significant difference (P
ISSN:0093-691X
1879-3231
DOI:10.1016/S0093-691X(98)00126-5