papG Alleles of Escherichia coli Strains Causing First-Episode or Recurrent Acute Cystitis in Adult Women

The distribution of the three alleles of the P adhesin gene papG (classes I–III) was assessed among 74 Escherichia coli urine isolates from women with first-episode or recurrent cystitis, and papG genotype was compared with clinical origin, O serogroup, agglutination of Gal(α1–4)Gal-coated latex bea...

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Bibliographic Details
Published in:The Journal of infectious diseases 1998-01, Vol.177 (1), p.97-101
Main Authors: Johnson, James R., Russo, Thomas A., Brown, Jennifer J., Stapleton, Ann
Format: Article
Language:English
Subjects:
Adhesins, Escherichia coli - genetics
Adhesins, Escherichia coli - urine
Adult
Adults
Agglutination
Alleles
Bacterial diseases
Bacterial diseases of the urinary system
Biological and medical sciences
Cystitis
Cystitis - genetics
Cystitis - immunology
DNA probes
DNA, Bacterial - analysis
Electrophoresis, Gel, Pulsed-Field
Erythrocytes
Escherichia coli
Escherichia coli - classification
Escherichia coli - genetics
Escherichia coli - pathogenicity
Escherichia coli Infections - genetics
Escherichia coli Infections - urine
Female
Fimbriae Proteins
Galactose
Genes, Bacterial - physiology
Genotypes
Hemolysin Proteins - metabolism
Human bacterial diseases
Humans
Infectious diseases
Major Articles
Medical sciences
O Antigens - analysis
Phenotypes
Polymerase chain reaction
Recurrence
Serotyping
Virulence - genetics
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Summary:The distribution of the three alleles of the P adhesin gene papG (classes I–III) was assessed among 74 Escherichia coli urine isolates from women with first-episode or recurrent cystitis, and papG genotype was compared with clinical origin, O serogroup, agglutination of Gal(α1–4)Gal-coated latex beads and human or sheep erythrocytes, and hemolysin production. The class-III-only papG genotype (27% of strains) predominated over the I + III (3%) and II-only (7%) genotypes, irrespective of clinical category. In contrast to the class II papG allele, the class III allele was significantly concentrated in serogroups O6 and O18. Agglutination phenotypes corresponded significantly but incompletely with papG genotype, whereas hemolysin production and papG positivity were tightly correlated. These findings suggest that in acute cystitis in adult women, the class III papG allele predominates, confers distinctive agglutination phenotypes, and is restricted to specific E. coli lineages.
ISSN:0022-1899
1537-6613
DOI:10.1086/513824