Expression of cell division cycle 2 kinase transcription in chronically rejected cardiac allografts of nonhuman primates

Accurate diagnosis and prevention of graft arteriosclerosis, known as chronic rejection, is critical to the success of cardiac transplantation, but often is difficult to attain. Expression of cell division cycle (cdc) 2 kinase, which plays a critical role in cell transition through the G2/M phase, i...

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Bibliographic Details
Published in:Heart and vessels 1997-01, Vol.12 (6), p.275-279
Main Authors: Schoenbeck, A, Isobe, M, Suzuki, J, Kato, M, Kitazawa, N, Amano, J, Endoh, M, Kawauchi, M, Takamoto, S, Sekiguchi, M
Format: Article
Language:English
Subjects:
Allografts
Animals
Arteries
Arteriosclerosis
Cdc2 protein
CDC2 Protein Kinase - genetics
CDC2 Protein Kinase - metabolism
Cell Cycle - genetics
Cell division
Cell proliferation
Coronary artery
Coronary Vessels - pathology
DNA Primers - chemistry
Gene Expression
Gene therapy
Genetic Therapy
Graft rejection
Graft Rejection - enzymology
Graft Rejection - pathology
Graft Rejection - prevention & control
Heart Transplantation - pathology
Heart transplants
Kinases
Macaca
Male
Muscles
Polymerase chain reaction
Prevention
Rejection
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - metabolism
RNA-directed DNA polymerase
Smooth muscle
Transplantation
Vascular diseases
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Summary:Accurate diagnosis and prevention of graft arteriosclerosis, known as chronic rejection, is critical to the success of cardiac transplantation, but often is difficult to attain. Expression of cell division cycle (cdc) 2 kinase, which plays a critical role in cell transition through the G2/M phase, is critical to the proliferation of vascular smooth muscle cells. To evaluate the usefulness of cdc2 kinase expression for pathophysiological analysis of chronic rejection, heterotopic cardiac transplantation was performed in Japanese monkeys (n = 7). Standard reverse transcriptase-polymerase chain reaction (RT-PCR) and in situ RT-PCR were performed to evaluate the expression. In the coronary arteries of chronically rejected allografts, enhanced cdc2 kinase expression was observed in thickened intima and media, while none was expressed in native hearts. The cdc2 kinase was also expressed before the intimal thickening occurred. These results indicate that enhanced expression of cdc2 kinase is a sensitive indicator for chronic cardiac rejection; targeting cdc2 kinase may be a viable gene therapy for prevention of this vasculopathy.
ISSN:0910-8327
1615-2573
DOI:10.1007/BF02766803