The relationship between microbial metabolic activity and biocorrosion of carbon steel

• Published in: Research in microbiology 1997-12, Vol.148 (9), p.785-793
• Main Authors: Dzierzewicz, Z., Cwalina, B., Chodurek, E., Wilczok, T.
• Format: Article
• Language: English
• Subjects: Acier; APS-reductase; APS-réductase; Biodeterioration. Biofouling; Biological and medical sciences; Biotechnology; Corrosion; Desulfovibrio - drug effects; Desulfovibrio - growth & development; Desulfovibrio - metabolism; Desulfovibrio desulfuricans; Dose-Response Relationship, Drug; Fundamental and applied biological sciences. Psychology; Hydrogen Sulfide - metabolism; Hydrogenase; Hydrogenase - metabolism; Hydrogénase; Industrial applications and implications. Economical aspects; Ions métalliques; Metal ions; Metals - administration & dosage; Metals - pharmacology; Oxidoreductases - metabolism; Oxidoreductases Acting on Sulfur Group Donors; Steel
• ISSN: 0923-2508; 1769-7123
• DOI: 10.1016/S0923-2508(97)82454-0

The effect of metabolic activity (expressed by generation time, rate of H 2S production and the activity of hydrogenase and adenosine phosphosulphate (APS)-reductase enzymes) of the 8 wild strains of Desulfovibrio desulfuricans and of their resistance to metal ions (Hg 2+, Cu 2+, Mn 2+, Zn 2+, Ni 2+, Cr 3+) on the rate of corrosion of carbon steel was studied. The medium containing lactate as the carbon source and sulphate as the electron acceptor was used for bacterial metabolic activity examination and in corrosive assays. Bacterial growth inhibition by metal ions was investigated in the sulphate-free medium. The rate of H 2S production was approximately directly proportional to the specific activities of the investigated enzymes. These activities were inversely proportional to the generation time. The rate of microbiologically induced corrosion (MIC) of carbon steel was directly proportional to bacterial resistance to metal ions (correlation coefficient r = 0.95). The correlation between the MIC rate and the activity of enzymes tested, although weaker, was also observed (r = 0.41 for APS-reductase; r = 0.69 for hydrogenase; critical value r c = 0.30, p = 0.05, n = 40). Nous avons étudié l'influence, sur le taux de corrosion de l'acier carboné, de l'activité métabolique (durée du cycle de reproduction, taux de production d'H 2S et activité de l'hydrogénase et de l'adénosinephosphosulfate (APS)-réductase) de 8 souches de Desulfovibrio desulfuricans, et de la résistance de ces souches aux ions métalliques (Hg 2+, Cu 2+, Mn 2+, Zn 2+, Ni 2+, Cr 3+). Le milieu contenant le lactate comme source de carbone et le sulfate comme accepteur d'électron a été utilisé pour l'étude du métabolisme bactérien et pour les tests de corrosion. L'inhibition de la croissance bactérienne par les ions métalliques a été étudiée en milieu sans sulfate. Le taux de production d'H 2S est approximativement directement proportionnel aux activités spécifiques des enzymes étudiées. Ces activités sont inversement proportionnelles à la durée du cycle de reproduction. Le taux de corrosion biologique (MIC, microbiologically induced corrosion) de l'acier carboné est directement proportionnel à la résistance bactérienne aux ions métalliques (coefficient de corrélation r = 0,95). Une corrélation entre les taux de MIC et ceux de l'activité enzymatique, bien que plus faible, a été aussi observée (r = 0,41 pour l'APS-réductase; r = 0,69 pour l'hydrogénase; valeur critique r = 0,30; p = 0,05; n = 4