Partial processing of the neuropeptide Y precursor in transfected CHO cells

The activation of regulatory peptides by post-translational modification of their biosynthetic precursors is generally thought to occur only in neuroendocrine cells. We have selected clones of Chinese hamster ovary cells, a non-neuroendocrine cell line, which were transfected with a eukaryotic expre...

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Bibliographic Details
Published in:FEBS letters 1990-02, Vol.261 (1), p.101-105
Main Authors: Wulff, Birgitte Schjellerup, O'Hare, Mairead M.T., Boel, Esper, Theill, Lars E., Schwartz, Thue W.
Format: Article
Language:English
Subjects:
Aminoacids, peptides. Hormones. Neuropeptides
Analytical, structural and metabolic biochemistry
Animals
Biological and medical sciences
Cell Line
CHO cells
Chromatography, Gel
Cloning, Molecular
Cricetinae
Fundamental and applied biological sciences. Psychology
Genetic Vectors
Humans
Neuroblastoma - analysis
Neuropeptide Y
Neuropeptide Y - genetics
Neuropeptide Y - metabolism
Peptide amidation
Peptide processing
Protein Precursors - genetics
Protein Precursors - metabolism
Protein Processing, Post-Translational
Proteins
Transfection
Tumor Cells, Cultured
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Summary:The activation of regulatory peptides by post-translational modification of their biosynthetic precursors is generally thought to occur only in neuroendocrine cells. We have selected clones of Chinese hamster ovary cells, a non-neuroendocrine cell line, which were transfected with a eukaryotic expression vector coding for the precursor for neuropeptide Y. Although the majority of the immunoreactive NPY was found in the form of pro-NPY, some degree of intracellular proteolytic processing of the precursor occurred in all clones. Part of the intracellular NPYimmunoreactivity was even correctly amidated. Extracellular degradation of pro-NPY in the tissue culture medium generated immunoreactivity which corresponded in size to NPY. It is concluded that precursor processing can occur in non-neuroendocrine cells both as a biological process within the cells and as apparent processing, degradation in the tissue culture medium.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(90)80646-Z