Maize high mobility group proteins bind to CCAAT and TATA boxes of a zein gene promoter

A 216-base pair promoter fragment of the 19-kDa zein storage protein gene pMS1, containing the CCAAT and TATA boxes, was analyzed for its in vitro interactions with purified high mobility group (HMG) proteins from maize endosperm tissue. It was found that the HMG proteins display a preferential bind...

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Bibliographic Details
Published in:The Journal of biological chemistry 1990-03, Vol.265 (8), p.4185-4188
Main Authors: Grasser, K D, Maier, U G, Haass, M M, Feix, G
Format: Article
Language:English
Subjects:
Base Sequence
Binding Sites
Deoxyribonuclease I
DNA - metabolism
DNA Restriction Enzymes
High Mobility Group Proteins - metabolism
Molecular Sequence Data
Molecular Weight
Phosphorylation
Promoter Regions, Genetic
Zea mays
Zea mays - analysis
Zein - genetics
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Summary:A 216-base pair promoter fragment of the 19-kDa zein storage protein gene pMS1, containing the CCAAT and TATA boxes, was analyzed for its in vitro interactions with purified high mobility group (HMG) proteins from maize endosperm tissue. It was found that the HMG proteins display a preferential binding of A/T-rich DNA regions like their animal counterparts, although the A/T content seems not to be the only determinant for binding specificity. Surprisingly, a specific binding of the HMG proteins occurs at the CCAAT and the TATA boxes as indicated by footprinting experiments.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(19)39542-0