Adaptation of a thermospray liquid chromatography/mass spectrometry interface for use with alkaline anion exchange liquid chromatography of carbohydrates

An interface is described that allows the direct coupling of high-performance alkaline anion exchange liquid chromatography with thermospray mass spectrometry. A membrane suppressor is used to remove nonvolatile alkaline salts from the mobile phase after the chromatographic process is completed and...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 1990-02, Vol.62 (3), p.248-252
Main Authors: Simpson, Richard C., Fenselau, Catherine C., Hardy, Mark R., Townsend, R. Reid, Lee, Yuan C., Cotter, Robert J.
Format: Article
Language:English
Subjects:
Analytical chemistry
Carbohydrates - analysis
Chemistry
Chromatographic methods and physical methods associated with chromatography
Chromatography, Ion Exchange - methods
Chromatography, Liquid
Exact sciences and technology
Gas chromatographic methods
Mass Spectrometry
Spectrophotometry, Ultraviolet
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Summary:An interface is described that allows the direct coupling of high-performance alkaline anion exchange liquid chromatography with thermospray mass spectrometry. A membrane suppressor is used to remove nonvolatile alkaline salts from the mobile phase after the chromatographic process is completed and prior to introduction into the mass spectrometer. Examples are given of both isocratic and gradient separations of a three-component test mixture of N-acetylated mono- and disaccharides, followed by on-line mass spectral data acquisition. Sensitivity studies show minimum detection limits for the test compounds to be in the microgram range.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac00202a005