Application of countercurrent chromatography/thermospray mass spectrometry for the identification of bioactive lignans from plant natural products

The versatility and resolving power of countercurrent chromatography (CCC) has been demonstrated with a newly developed analytical high-speed planet centrifuge system. Interfacing countercurrent chromatography with mass spectrometry (MS) provides a new analytical methodology that integrates the adva...

Full description

Saved in:
Bibliographic Details
Published in:Analytical chemistry (Washington) 1990-02, Vol.62 (3), p.244-248
Main Authors: Lee, Yue Wei, Voyksner, Robert D., Pack, Terry W., Cook, C. Edgar, Fang, Q. C., Ito, Yoichiro
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Chromatography - methods
Fundamental and applied biological sciences. Psychology
Intermediary metabolites. Miscellaneous
Lignans
Lignin - analysis
Mass Spectrometry
Other biological molecules
Plants - analysis
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The versatility and resolving power of countercurrent chromatography (CCC) has been demonstrated with a newly developed analytical high-speed planet centrifuge system. Interfacing countercurrent chromatography with mass spectrometry (MS) provides a new analytical methodology that integrates the advantages of countercurrent chromatography with the low detection limit and identification capability of mass spectrometry. In this paper the capability of thermospray CCC/MS is demonstrated in identifying and validating the bioactive and structurally known lignans from a crude extract of Schisandra rubriflora Rhed et Wils, a traditional Chinese herbal medicine for treatment of hepatitis.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac00202a004