Retaining and recovering enzyme activity during degradation of TCE by methanotrophs

To determine if compounds added during trichloroethylene (TCE) degradation could reduce the loss of enzyme activity or increase enzyme recovery, different compounds serving as energy and carbon sources, pH buffers, or free radical scavengers were tested. Formate and formic acid (reducing power and a...

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Bibliographic Details
Published in:Applied biochemistry and biotechnology 1997, Vol.63-65 (1), p.789-796
Main Authors: PALUMBO, A. V, STRONG-GUNDERSON, J. M, CARROLL, S
Format: Article
Language:English
Subjects:
Bacteria
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
Calcium carbonate
Carbon sources
Enzymatic activity
Fundamental and applied biological sciences. Psychology
Microbiology
Mission oriented research
Physiology and metabolism
Scavengers
Solvents
Trichloroethene
Trichloroethylene
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Summary:To determine if compounds added during trichloroethylene (TCE) degradation could reduce the loss of enzyme activity or increase enzyme recovery, different compounds serving as energy and carbon sources, pH buffers, or free radical scavengers were tested. Formate and formic acid (reducing power and a carbon source), as well as ascorbic acid and citric acid (free radical scavengers) were added during TCE degradation at a concentration of 2 mM. A saturated solution of calcium carbonate was also tested to address pH concerns. In the presence of formate and methane, only calcium carbonate and formic acid had a beneficial effect on enzyme recovery. The calcium carbonate and formic acid both reduced the loss of enzyme activity and resulted in the highest levels of enzyme activity after recovery.
ISSN:0273-2289
1559-0291
DOI:10.1007/BF02920475