Transcriptional activation of the human villin gene during enterocyte differentiation

Enterocyte differentiation occurs along the crypt-villus axis and is generally thought to involve the transcriptional activation of cell-specific genes, among which is the brush-border structural protein villin. We have examined the molecular mechanisms of villin induction using both in vivo and in...

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Bibliographic Details
Published in:Journal of gastrointestinal surgery 1997-09, Vol.1 (5), p.433-438
Main Authors: Hodin, Richard A., Shei, Amy, Meng, Shufen
Format: Article
Language:English
Subjects:
Animals
Cell Differentiation
Cells
Cells, Cultured
Enterocytes - cytology
Gene expression
Humans
Kinases
Microfilament Proteins - genetics
Proteins
Rats
Rats, Sprague-Dawley
RNA, Messenger - biosynthesis
Transcription, Genetic - physiology
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Summary:Enterocyte differentiation occurs along the crypt-villus axis and is generally thought to involve the transcriptional activation of cell-specific genes, among which is the brush-border structural protein villin. We have examined the molecular mechanisms of villin induction using both in vivo and in vitro systems. Total RNA was purified from rat tissues or cultured cells by the guanidinium thiocyanate method and Northern blot analyses carried out using radiolabeled complementary DNA probes specific for villin or the actin control. Transient transfection (calcium/phosphate method) assays were performed using a luciferase reporter gene containing 2 kb of the human villin gene 5′-flanking region. We have found that the villin mRNA was expressed at high levels in the small intestine, to a lesser degree in the colon, and was not detected in the brain or liver. In HT-29 cells, villin mRNA levels increased 2.5-fold ( P
ISSN:1091-255X
1873-4626
DOI:10.1016/S1091-255X(97)80130-8