PRODUCTION, ISOLATION AND STRUCTURE DETERMINATION OF A NOVEL β-GLUCOSIDASE INHIBITOR, CYCLOPHELLITOL, FROM PHELLINUS SP

In the course of our screening of β-glucosidase inhibitor, a culture filtrate of a mushroom, Phellinus sp. strongly inhibited the enzyme activity. The active substance was isolated through charcoal separation, column chromatography and crystallization. Spectroscopic and crystallographic analysis rev...

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Published in:Journal of antibiotics 1990/01/25, Vol.43(1), pp.49-53
Main Authors: ATSUMI, SONOKO, UMEZAWA, KAZUO, IINUMA, HIRONOBU, NAGANAWA, HIROSHI, NAKAMURA, HIKARU, IITAKA, YOICHI, TAKEUCHI, TOMIO
Format: Article
Language:English
Subjects:
Antibiotics, microbial producers, chemotherapic agents, antiseptics, disinfecting agents
Applied microbiology
Basidiomycota - metabolism
beta-Glucosidase - antagonists & inhibitors
Biological and medical sciences
Chemotherapic agents
Crystallography
Cyclohexanols - analysis
Cyclohexanols - pharmacology
Fermentation
Fundamental and applied biological sciences. Psychology
Glucosidases - antagonists & inhibitors
Microbiology
Molecular Structure
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Summary:In the course of our screening of β-glucosidase inhibitor, a culture filtrate of a mushroom, Phellinus sp. strongly inhibited the enzyme activity. The active substance was isolated through charcoal separation, column chromatography and crystallization. Spectroscopic and crystallographic analysis revealed that it had a novel cyclitol structure, (1S, 2R, 3S, 4R, 5R, 6R)-5-hydroxymeihy¥-loxabicyclo[4, l, 0]heptane-2, 3, 4-triol, and we named it cyclophellitol. It inhibited almond-derived β-glucosidase with an IC50 of 0.8μg/ml.
ISSN:0021-8820
1881-1469
DOI:10.7164/antibiotics.43.49