In vivo transfection of melanoma cells by lithotripter shock waves

The potential for gene transfection during shock wave tumor therapy was evaluated by searching for shock wave-induced DNA transfer in mouse tumor cells. B16 mouse melanoma cells were cultured by standard methods and implanted s.c. in female C57BL/6 mice 10-14 days before treatment. A luciferase repo...

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Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 1998-01, Vol.58 (2), p.219-221
Main Authors: BAO, S, THRALL, B. D, GIES, R. A, MILLER, D. L
Format: Article
Language:English
Subjects:
Animals
Antineoplastic agents
Biological and medical sciences
Cell Survival - radiation effects
Escherichia coli - enzymology
Escherichia coli - genetics
Female
General aspects
Genes, Reporter
Genetic Vectors
Lithotripsy - methods
Luciferases - biosynthesis
Luciferases - genetics
Medical sciences
Melanoma, Experimental - enzymology
Melanoma, Experimental - genetics
Mice
Mice, Inbred C57BL
Neoplasm Transplantation
Pharmacology. Drug treatments
Plasmids
Transfection - methods
Tumor Cells, Cultured
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Summary:The potential for gene transfection during shock wave tumor therapy was evaluated by searching for shock wave-induced DNA transfer in mouse tumor cells. B16 mouse melanoma cells were cultured by standard methods and implanted s.c. in female C57BL/6 mice 10-14 days before treatment. A luciferase reporter vector was used as the DNA plasmid for intratumoral injection at 0.2 mg/ml tumor. Air at 10% of tumor volume was injected after the DNA in some tumors to enhance acoustic cavitation activity. The shock wave generation system was similar to a Dornier HM-3 lithotripter with pressure amplitudes of 24.4 MPa peak positive and 5.2 MPa peak negative. Luciferase production in isolated tumor cells was measured with a luminometer 1 day after treatment to assess gene transfer and expression. Exposure to 800 shock waves, followed by immediate isolation and culture of tumor cells for 1 day, yielded 1.1 (0.43 SE) pg/10(6) cells for plasmid injection only and 7.5 (2.5 SE) pg/10(6) cells for plasmid plus air injection. Significantly increased luciferase production, relative to shams, occurred for 200-, 400-, 800-, and 1200-shock wave treatments with plasmid and air injection. Exposure with the isolation of tumor cells delayed for a day to allow gene expression within the growing tumors gave increased luciferase production for 100- and 400-shock wave exposures without and with air injection. Gene transfer therefore can be induced during lithotripter shock wave treatment in vivo, particularly with enhanced acoustic cavitation, which supports the concept that gene and shock wave therapy might be advantageously merged.
ISSN:0008-5472
1538-7445