Enhanced activation of platelets with abnormal release of RANTES in human immunodeficiency virus type 1 infection

• Published in: The FASEB journal 1998-01, Vol.12 (1), p.79-90
• Main Authors: Holme, P A, Müller, F, Solum, N O, Brosstad, F, Frøland, S S, Aukrust, P
• Format: Article
• Language: English
• Subjects: AIDS/HIV; Anti-HIV Agents - therapeutic use; Biomarkers; Chemokine CCL5 - metabolism; Flow Cytometry; HIV Infections - drug therapy; HIV Infections - immunology; HIV-1; Humans; Platelet Activation - immunology; Viral Load
• ISSN: 0892-6638; 1530-6860
• DOI: 10.1096/fasebj.12.1.79

Besides their role in hemostasis, platelets are involved in inflammatory and immunological processes, and we hypothesize that platelet activation may play an immunopathogenetic role in HIV-1 infection. Blood was drawn from 15 controls and 20 HIV-1-infected patients with normal platelet counts, classified into groups of non-AIDS and AIDS. Platelet activation was detected using flow cytometry with mAbs against the release markers P-selectin and CD63, mAb against GPIb, and the probe annexin V detecting surface exposure of aminophospholipids. The amount of microvesicles was measured using mAb against GPIIIa. Compared to controls, blood samples from HIV-1-infected patients showed significantly enhanced levels of microvesicles and activated platelets as detected by their exposure of P-selectin, CD63, and aminophospholipids, as well as reduction in GPIb expression. Increased expression of P-selectin and amounts of microvesicles were most pronounced in advanced clinical and immunological disease. When studying the effect of HIV-1 protease inhibitor therapy (indinavir) on platelet activation, we found that concomitant with a profound decrease in plasma viral load, there was a near normalization of several of the parameters reflecting enhanced platelet activation. Finally, we demonstrated that platelets may be an important source of the chemokine RANTES in HIV-1-infected patients. Although both unstimulated and SFLLRN-stimulated platelets from asymptomatic patients had enhanced release of RANTES, platelets from AIDS patients were characterized by markedly enhanced spontaneous, but decreased SFLLRN-stimulated release of this chemokine. Taken together, these results, which demonstrate for the first time increased platelet activation in HIV-1-infected patients with normal platelet counts, may represent a previously unrecognized immunopathogenic factor in HIV-1 infection.