Potent inhibition of human ovarian steroidogenesis by insulin-like growth factor binding protein-4 (IGFBP-4)

Several studies have now documented the existence of IGFBPs in follicular fluid and their correlation with the health of the follicle. In particular, increased levels of IGFBP-4 have been reported in androgen-dominant atretic follicles and those from polycystic ovaries. The aim of this study was to...

Full description

Saved in:
Bibliographic Details
Published in:The journal of clinical endocrinology and metabolism 1998, Vol.83 (1), p.284-287
Main Authors: MASON, H. D, CWYFAN-HUGHES, S, HOLLY, J. M. P, FRANKS, S
Format: Article
Language:English
Subjects:
Androstenedione - biosynthesis
Biological and medical sciences
Cells, Cultured
Estradiol - biosynthesis
Female
Follicle Stimulating Hormone - antagonists & inhibitors
Follicle Stimulating Hormone - pharmacology
Fundamental and applied biological sciences. Psychology
Granulosa Cells - drug effects
Granulosa Cells - metabolism
Hormone metabolism and regulation
Humans
Insulin-Like Growth Factor Binding Protein 4 - pharmacology
Luteinizing Hormone - antagonists & inhibitors
Luteinizing Hormone - pharmacology
Mammalian female genital system
Theca Cells - drug effects
Theca Cells - metabolism
Vertebrates: reproduction
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Several studies have now documented the existence of IGFBPs in follicular fluid and their correlation with the health of the follicle. In particular, increased levels of IGFBP-4 have been reported in androgen-dominant atretic follicles and those from polycystic ovaries. The aim of this study was to elucidate the role of IGFBP-4 in ovarian steroidogenesis. Granulosa cells and theca tissue were incubated with or without LH or FSH in the presence or absence of IGFBP-4 (0.5-50 ng/ml). Inhibition by IGFBP-4 of estradiol production in the presence of testosterone alone was seen in three of four experiments. IGFBP-4 completely inhibited FSH-stimulated estradiol production in three experiments and caused 67% inhibition in a fourth. Similar results were obtained for theca, in which concurrent incubation with IGFBP-4 completely negated the stimulatory effects of LH on androstenedione production. The mechanism by which IGFBP-4 exerts these potent effects and the possibility that this may by IGF-independent are currently being investigated.
ISSN:0021-972X
1945-7197
DOI:10.1210/jc.83.1.284