Superoxide Release is Involved in Membrane Potential Changes in Mouse Peritoneal Macrophages

Participation of reactive oxygen species (ROS) in the changes in macrophage membrane potential resulted from effects of different agonists has been studied. Treatment of macrophages with chemotactic peptide fMLP or platelet-activating factor (PAF) caused a brief depolarization followed by a long-las...

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Bibliographic Details
Published in:Free radical biology & medicine 1998, Vol.24 (1), p.168-174
Main Authors: Gamaley, Irene A., Kirpichnikova, Ksenia M., Klyubin, Igor V.
Format: Article
Language:English
Subjects:
Animals
Chemiluminescence
Free radicals
Intracellular Ca 2
K + channels
Luminescent Measurements
Macrophages, Peritoneal - metabolism
Membrane potential
Membrane Potentials - physiology
Mice
N-Formylmethionine Leucyl-Phenylalanine - pharmacology
Oxonol dye
Platelet Activating Factor - pharmacology
Reactive Oxygen Species - metabolism
Respiratory Burst - physiology
Superoxide
Superoxides - metabolism
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Summary:Participation of reactive oxygen species (ROS) in the changes in macrophage membrane potential resulted from effects of different agonists has been studied. Treatment of macrophages with chemotactic peptide fMLP or platelet-activating factor (PAF) caused a brief depolarization followed by a long-lasting hyperpolarization. Lipopolysaccharide and interferon-γ only depolarized the plasma membrane. Chemiluminescence measurements indicated that only fMLP and PAF activated macrophages to release ROS. The hyperpolarization response of the cell was significantly decreased in the presence of superoxide dismutase (but not catalase). Moreover, the O 2   ̇ − -generating system, xanthine plus xanthine oxidase, caused a marked hyperpolarization. In all the cases, the hyperpolarization induced by fMLP, PAF and O 2   ̇ − -generating system was found to depend on the concentration of intracellular Ca 2+ and extracellular K +. Furthermore, in the presence of quinidine, a blocker of Ca 2+-dependent K + conductance fMLP and PAF caused only prolonged depolarization while the effect of O 2   ̇ − was reduced to a minimum. These data suggest that the macrophage hyperpolarization response to fMLP and PAF involves superoxide-mediated Ca 2+-dependent alteration of the relative membrane permeability to K +.
ISSN:0891-5849
1873-4596
DOI:10.1016/S0891-5849(97)00212-8