Identification and characterization of a protein kinase-interacting protein encoded by the Autographa californica nuclear polyhedrosis virus

We have used a yeast two-hybrid system to identify a baculoviral gene encoding a protein kinase-interacting protein (PKIP). The pkip gene is located at 15.8-16.2 m.u. (map units) and represents ORF 24 in the sequence of Ayres et al. (1994) of the Autographa californica nuclear polyhedrosis virus. PK...

Full description

Saved in:
Bibliographic Details
Published in:Virology (New York, N.Y.) N.Y.), 1998-01, Vol.240 (2), p.175-182
Main Authors: Fan, X. (University of Pennsylvania, Philadelphia, PA.), McLachlin, J.R, Weaver, R.F
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
AUTOGRAPHA CALIFORNICA
Base Sequence
Blotting, Northern
Carrier Proteins - chemistry
Carrier Proteins - isolation & purification
Enzyme Activation
Genetic Vectors
Molecular Sequence Data
NUCLEAR POLYHEDROSIS VIRUS
Nucleopolyhedrovirus - genetics
Sequence Alignment
Spodoptera - virology
Viral Proteins - metabolism
VIRUS POLIEDROSIS NUCLEAR
VIRUS POLYEDROSE NUCLEAIRE
Yeasts - genetics
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We have used a yeast two-hybrid system to identify a baculoviral gene encoding a protein kinase-interacting protein (PKIP). The pkip gene is located at 15.8-16.2 m.u. (map units) and represents ORF 24 in the sequence of Ayres et al. (1994) of the Autographa californica nuclear polyhedrosis virus. PKIP is a 19.2-kDa protein and appears to stimulate the activity of the viral protein kinase-1 in vitro. Northern blotting analysis revealed that pkip is a late gene. Attempts to plaque purify a pkip(-) virus were unsuccessful
ISSN:0042-6822
1096-0341
DOI:10.1006/viro.1997.8944