Chlorella Virus NY-2A Encodes at Least 12 DNA Endonuclease/Methyltransferase Genes

The 380-kb chlorella virus NY-2A genome is highly methylated; 45% of the cytosines are 5-methylcytosine (5mC) and 37% of the adenines are N6-methyladenine (6mA). Based on the sensitivity/resistance of NY-2A DNA to 80 methylation-sensitive DNA restriction endonucleases, the virus is predicted to enco...

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Published in:Virology (New York, N.Y.) N.Y.), 1998-01, Vol.240 (2), p.366-375
Main Authors: Zhang, Yanping, Nelson, Michael, Nietfeldt, Joe, Xia, Yuannan, Burbank, Dwight, Ropp, Susan, Van Etten, James L.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
AMINO ACID SEQUENCES
CHEMICAL COMPOSITION
CHLORELLA
Chlorella - virology
COMPOSICION QUIMICA
COMPOSITION CHIMIQUE
Deoxyribonuclease I - genetics
Deoxyribonuclease I - isolation & purification
DNA Methylation
DNA Modification Methylases - genetics
DNA Modification Methylases - isolation & purification
GENBANK/AF021248
GENBANK/AF021249
GENBANK/AF021250
GENBANK/AF021251
GENBANK/AF021252
Genes, Viral - genetics
MOLECULAR SEQUENCE DATA
Phycodnaviridae - enzymology
Phycodnaviridae - genetics
PLANT VIRUSES
Sequence Alignment
Sequence Homology, Amino Acid
VIRUS DE LAS PLANTAS
VIRUS DES VEGETAUX
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Summary:The 380-kb chlorella virus NY-2A genome is highly methylated; 45% of the cytosines are 5-methylcytosine (5mC) and 37% of the adenines are N6-methyladenine (6mA). Based on the sensitivity/resistance of NY-2A DNA to 80 methylation-sensitive DNA restriction endonucleases, the virus is predicted to encode at least 10 DNA methyltransferases: 7 6mA-specific methyltransferases, M.CviQI (GTmAC), M.CvQII (RmAR), M.CviQIII (TCGmA), M.CviQIV (GmATC), M.CviQV (TGCmA), M.CviQVI (GmANTC), and M.CviQVII (CmATG); and 3 5mC-specific methyltransferases, M.CviQVIII [RGmC(T/C/G)], M.CviQIX (mCC), and M.CviQX (mCGR). Five of the 6mA methyltransferase genes,M.CviQI, M.CviQIII, M.CviQV, M.CviQVI,andM.CviQVII,were cloned and sequenced. In addition, 2 site-specific endonuclease activities, R.CviQI (G/TAC) and NY2A-nickase (R/AG), were detected in cell-free extracts from NY-2A virus-infected chlorella. Therefore, the NY-2A genome contains at least 12 DNA methyltransferase and endonuclease genes which, altogether, compose about 3–4% of the virus genome.
ISSN:0042-6822
1096-0341
DOI:10.1006/viro.1997.8936