Identification of a protein essential for replication of porcine circovirus

The largest open reading frame of porcine circovirus (ORF4) encodes a protein of 312 amino acids. The predicted gene product of ORF 4 shows similarities to Rep proteins of other plant circo-viruses and geminiviruses. Three motifs have been identified that are characteristic for proteins involved in...

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Bibliographic Details
Published in:Journal of general virology 1998-02, Vol.79 (2), p.381-384
Main Authors: Mankertz, A, Mankertz, J, Wolf, K, Buhk, H.J
Format: Article
Language:English
Subjects:
Amino Acid Sequence
amino acid sequences
Animals
Base Sequence
Cell Line
Circovirus - physiology
DNA Helicases - chemistry
DNA Helicases - genetics
DNA Helicases - metabolism
DNA-Binding Proteins
Molecular Sequence Data
nucleotide sequences
Open Reading Frames
Recombinant Proteins - biosynthesis
Swine
Trans-Activators - chemistry
Trans-Activators - genetics
Trans-Activators - metabolism
Transfection
viral proteins
Virus Replication
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Description
Summary:The largest open reading frame of porcine circovirus (ORF4) encodes a protein of 312 amino acids. The predicted gene product of ORF 4 shows similarities to Rep proteins of other plant circo-viruses and geminiviruses. Three motifs have been identified that are characteristic for proteins involved in rolling circle replication and the consensus sequence for a putative dNTP-binding box (GKS) has been found. In this paper, experimental evidence is presented which indicates that ORF 4 encodes the replication protein of porcine circo-virus. After cloning of the ORF 4 gene product, it was supplied in trans in a transient replication assay. The ORF 4 gene product promoted the replication of plasmid pOP11, which carries the origin of DNA replication of porcine circovirus. Since pOP11 itself is unable to replicate in virus-free porcine kidney cells, the ORF 4 gene product must be essential for replication of porcine circovirus.
ISSN:0022-1317
1465-2099
DOI:10.1099/0022-1317-79-2-381