Loading…
α 1-Adrenergic stimulation of Cl − efflux in isolated brown adipocytes
Unidirectional 36Cl − efflux from preloaded isolated brown adipocytes was studied. A norepinephrine-stimulated 36Cl − efflux pathway was found which approximately doubled the rate of 36Cl − efflux from the cells. The response to norepinephrine was fully inhibited by the α 1-adrenergic antagonist pra...
Saved in:
| Published in: | FEBS letters 1990-03, Vol.262 (1), p.25-28 |
|---|---|
| Main Authors: | , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c289t-370d6a6c9c7acc317ea61fb1c7027ca2e0db43b6a63ed45947c2da64b10df5fd3 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c289t-370d6a6c9c7acc317ea61fb1c7027ca2e0db43b6a63ed45947c2da64b10df5fd3 |
| container_end_page | 28 |
| container_issue | 1 |
| container_start_page | 25 |
| container_title | FEBS letters |
| container_volume | 262 |
| creator | Dasso, Leonardo Connolly, Eamonn Nedergaard, Jan |
| description | Unidirectional
36Cl
− efflux from preloaded isolated brown adipocytes was studied. A norepinephrine-stimulated
36Cl
− efflux pathway was found which approximately doubled the rate of
36Cl
− efflux from the cells. The response to norepinephrine was fully inhibited by the α
1-adrenergic antagonist prazosin, but was unaffected by the β-adrenergic antagonist propranolol, showing that norepinephrine stimulated the
36Cl
− efflux pathway via the α
1-adrenoceptor. The stimulation of
36Cl
− efflux could not be mimicked by the Ca
2+ ionophore A23187, indicating that the effect was not mediated by elevation of the intracellular Ca
2+ level. It is concluded that brown fat cells possess a specific mechanism for α
1-adrenergic stimulation of Cl
− efflux. The possibility is discussed that this Cl
− efflux pathway could be the basis for the early α-adrenergic depolarization seen in brown fat cells. |
| doi_str_mv | 10.1016/0014-5793(90)80144-8 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_79689319</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0014579390801448</els_id><sourcerecordid>79689319</sourcerecordid><originalsourceid>FETCH-LOGICAL-c289t-370d6a6c9c7acc317ea61fb1c7027ca2e0db43b6a63ed45947c2da64b10df5fd3</originalsourceid><addsrcrecordid>eNp9kE1OwzAQhS0EKqVwA5C8QrAI2HFixxukquKnUiU2sLYce4KM0rjYCdAbsOYmXIRDcBJSWnXJavT03pvRfAgdU3JBCeWXhNAsyYVkZ5KcF73IkmIHDWkhWMIyXuyi4Tayjw5ifCa9LqgcoEFKcy5YOkTT7y9Mk7EN0EB4cgbH1s27WrfON9hXeFLjn49PDFVVd-_YNdhF37tgcRn8W4O1dQtvli3EQ7RX6TrC0WaO0OPN9cPkLpnd304n41li0kK2CRPEcs2NNEIbw6gAzWlVUiNIKoxOgdgyY2UfYWCzXGbCpFbzrKTEVnll2Qidrvcugn_pILZq7qKButYN-C4qIXkhGZV9MFsHTfAxBqjUIri5DktFiVoRVCs8aoVHSaL-CKqir51s9nflHOy2tEHW-1drH_onXx0EFY2DxoB1AUyrrHf_H_gF_3OBUw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>79689319</pqid></control><display><type>article</type><title>α 1-Adrenergic stimulation of Cl − efflux in isolated brown adipocytes</title><source>ScienceDirect</source><creator>Dasso, Leonardo ; Connolly, Eamonn ; Nedergaard, Jan</creator><creatorcontrib>Dasso, Leonardo ; Connolly, Eamonn ; Nedergaard, Jan</creatorcontrib><description>Unidirectional
36Cl
− efflux from preloaded isolated brown adipocytes was studied. A norepinephrine-stimulated
36Cl
− efflux pathway was found which approximately doubled the rate of
36Cl
− efflux from the cells. The response to norepinephrine was fully inhibited by the α
1-adrenergic antagonist prazosin, but was unaffected by the β-adrenergic antagonist propranolol, showing that norepinephrine stimulated the
36Cl
− efflux pathway via the α
1-adrenoceptor. The stimulation of
36Cl
− efflux could not be mimicked by the Ca
2+ ionophore A23187, indicating that the effect was not mediated by elevation of the intracellular Ca
2+ level. It is concluded that brown fat cells possess a specific mechanism for α
1-adrenergic stimulation of Cl
− efflux. The possibility is discussed that this Cl
− efflux pathway could be the basis for the early α-adrenergic depolarization seen in brown fat cells.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/0014-5793(90)80144-8</identifier><identifier>PMID: 2156732</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Adipose Tissue, Brown - metabolism ; Animals ; Brown adipose tissue ; Calcium - physiology ; Chlorides - metabolism ; Cl − flux ; Cricetinae ; Hamster ; In Vitro Techniques ; Membrane depolarization ; Membrane Potentials ; Mesocricetus ; Nonshivering thermogenesis ; Norepinephrine - pharmacology ; Prazosin ; Receptors, Adrenergic, alpha - physiology</subject><ispartof>FEBS letters, 1990-03, Vol.262 (1), p.25-28</ispartof><rights>1990</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c289t-370d6a6c9c7acc317ea61fb1c7027ca2e0db43b6a63ed45947c2da64b10df5fd3</citedby><cites>FETCH-LOGICAL-c289t-370d6a6c9c7acc317ea61fb1c7027ca2e0db43b6a63ed45947c2da64b10df5fd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0014579390801448$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3549,27924,27925,45780</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2156732$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dasso, Leonardo</creatorcontrib><creatorcontrib>Connolly, Eamonn</creatorcontrib><creatorcontrib>Nedergaard, Jan</creatorcontrib><title>α 1-Adrenergic stimulation of Cl − efflux in isolated brown adipocytes</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>Unidirectional
36Cl
− efflux from preloaded isolated brown adipocytes was studied. A norepinephrine-stimulated
36Cl
− efflux pathway was found which approximately doubled the rate of
36Cl
− efflux from the cells. The response to norepinephrine was fully inhibited by the α
1-adrenergic antagonist prazosin, but was unaffected by the β-adrenergic antagonist propranolol, showing that norepinephrine stimulated the
36Cl
− efflux pathway via the α
1-adrenoceptor. The stimulation of
36Cl
− efflux could not be mimicked by the Ca
2+ ionophore A23187, indicating that the effect was not mediated by elevation of the intracellular Ca
2+ level. It is concluded that brown fat cells possess a specific mechanism for α
1-adrenergic stimulation of Cl
− efflux. The possibility is discussed that this Cl
− efflux pathway could be the basis for the early α-adrenergic depolarization seen in brown fat cells.</description><subject>Adipose Tissue, Brown - metabolism</subject><subject>Animals</subject><subject>Brown adipose tissue</subject><subject>Calcium - physiology</subject><subject>Chlorides - metabolism</subject><subject>Cl − flux</subject><subject>Cricetinae</subject><subject>Hamster</subject><subject>In Vitro Techniques</subject><subject>Membrane depolarization</subject><subject>Membrane Potentials</subject><subject>Mesocricetus</subject><subject>Nonshivering thermogenesis</subject><subject>Norepinephrine - pharmacology</subject><subject>Prazosin</subject><subject>Receptors, Adrenergic, alpha - physiology</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><recordid>eNp9kE1OwzAQhS0EKqVwA5C8QrAI2HFixxukquKnUiU2sLYce4KM0rjYCdAbsOYmXIRDcBJSWnXJavT03pvRfAgdU3JBCeWXhNAsyYVkZ5KcF73IkmIHDWkhWMIyXuyi4Tayjw5ifCa9LqgcoEFKcy5YOkTT7y9Mk7EN0EB4cgbH1s27WrfON9hXeFLjn49PDFVVd-_YNdhF37tgcRn8W4O1dQtvli3EQ7RX6TrC0WaO0OPN9cPkLpnd304n41li0kK2CRPEcs2NNEIbw6gAzWlVUiNIKoxOgdgyY2UfYWCzXGbCpFbzrKTEVnll2Qidrvcugn_pILZq7qKButYN-C4qIXkhGZV9MFsHTfAxBqjUIri5DktFiVoRVCs8aoVHSaL-CKqir51s9nflHOy2tEHW-1drH_onXx0EFY2DxoB1AUyrrHf_H_gF_3OBUw</recordid><startdate>19900312</startdate><enddate>19900312</enddate><creator>Dasso, Leonardo</creator><creator>Connolly, Eamonn</creator><creator>Nedergaard, Jan</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19900312</creationdate><title>α 1-Adrenergic stimulation of Cl − efflux in isolated brown adipocytes</title><author>Dasso, Leonardo ; Connolly, Eamonn ; Nedergaard, Jan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c289t-370d6a6c9c7acc317ea61fb1c7027ca2e0db43b6a63ed45947c2da64b10df5fd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Adipose Tissue, Brown - metabolism</topic><topic>Animals</topic><topic>Brown adipose tissue</topic><topic>Calcium - physiology</topic><topic>Chlorides - metabolism</topic><topic>Cl − flux</topic><topic>Cricetinae</topic><topic>Hamster</topic><topic>In Vitro Techniques</topic><topic>Membrane depolarization</topic><topic>Membrane Potentials</topic><topic>Mesocricetus</topic><topic>Nonshivering thermogenesis</topic><topic>Norepinephrine - pharmacology</topic><topic>Prazosin</topic><topic>Receptors, Adrenergic, alpha - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dasso, Leonardo</creatorcontrib><creatorcontrib>Connolly, Eamonn</creatorcontrib><creatorcontrib>Nedergaard, Jan</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dasso, Leonardo</au><au>Connolly, Eamonn</au><au>Nedergaard, Jan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>α 1-Adrenergic stimulation of Cl − efflux in isolated brown adipocytes</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1990-03-12</date><risdate>1990</risdate><volume>262</volume><issue>1</issue><spage>25</spage><epage>28</epage><pages>25-28</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>Unidirectional
36Cl
− efflux from preloaded isolated brown adipocytes was studied. A norepinephrine-stimulated
36Cl
− efflux pathway was found which approximately doubled the rate of
36Cl
− efflux from the cells. The response to norepinephrine was fully inhibited by the α
1-adrenergic antagonist prazosin, but was unaffected by the β-adrenergic antagonist propranolol, showing that norepinephrine stimulated the
36Cl
− efflux pathway via the α
1-adrenoceptor. The stimulation of
36Cl
− efflux could not be mimicked by the Ca
2+ ionophore A23187, indicating that the effect was not mediated by elevation of the intracellular Ca
2+ level. It is concluded that brown fat cells possess a specific mechanism for α
1-adrenergic stimulation of Cl
− efflux. The possibility is discussed that this Cl
− efflux pathway could be the basis for the early α-adrenergic depolarization seen in brown fat cells.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>2156732</pmid><doi>10.1016/0014-5793(90)80144-8</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0014-5793 |
| ispartof | FEBS letters, 1990-03, Vol.262 (1), p.25-28 |
| issn | 0014-5793 1873-3468 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_79689319 |
| source | ScienceDirect |
| subjects | Adipose Tissue, Brown - metabolism Animals Brown adipose tissue Calcium - physiology Chlorides - metabolism Cl − flux Cricetinae Hamster In Vitro Techniques Membrane depolarization Membrane Potentials Mesocricetus Nonshivering thermogenesis Norepinephrine - pharmacology Prazosin Receptors, Adrenergic, alpha - physiology |
| title | α 1-Adrenergic stimulation of Cl − efflux in isolated brown adipocytes |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-28T15%3A02%3A38IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=%CE%B1%201-Adrenergic%20stimulation%20of%20Cl%20%E2%88%92%20efflux%20in%20isolated%20brown%20adipocytes&rft.jtitle=FEBS%20letters&rft.au=Dasso,%20Leonardo&rft.date=1990-03-12&rft.volume=262&rft.issue=1&rft.spage=25&rft.epage=28&rft.pages=25-28&rft.issn=0014-5793&rft.eissn=1873-3468&rft_id=info:doi/10.1016/0014-5793(90)80144-8&rft_dat=%3Cproquest_cross%3E79689319%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c289t-370d6a6c9c7acc317ea61fb1c7027ca2e0db43b6a63ed45947c2da64b10df5fd3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=79689319&rft_id=info:pmid/2156732&rfr_iscdi=true |