Extraction of Octamethylcyclotetrasiloxane and Its Metabolites from Biological Matrices

Octamethylcyclotetrasiloxane (D4) is an industrial chemical of significant commercial importance. It is a key ingredient in a variety of product formulations and a critical intermediate in the production of silicone polymers. As part of the pharmacokinetic investigation of its disposition and metabo...

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Bibliographic Details
Published in:Analytical biochemistry 1998-02, Vol.256 (1), p.14-22
Main Authors: Varaprath, Sudarsanan, Salyers, Kevin L., Plotzke, Kathleen P., Nanavati, Shrenik
Format: Article
Language:English
Subjects:
Animals
Chromatography, High Pressure Liquid
Evaluation Studies as Topic
Furans
Gas Chromatography-Mass Spectrometry
Male
Rats
Rats, Inbred F344
Siloxanes - isolation & purification
Siloxanes - metabolism
Siloxanes - pharmacokinetics
Solvents
Tissue Distribution
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Summary:Octamethylcyclotetrasiloxane (D4) is an industrial chemical of significant commercial importance. It is a key ingredient in a variety of product formulations and a critical intermediate in the production of silicone polymers. As part of the pharmacokinetic investigation of its disposition and metabolism, an efficient extraction methodology has been developed to recover both the parent D4and its metabolites from various biological matrices, including blood, plasma, urine,feces, liver, lung, and fat. Of the common organicsolvents evaluated (hexane, methylene chloride, diethylether, chloroform, methylisobutylketone, etc.), tetrahydrofuran (THF) was demonstrated to be an excellent choice for extracting both D4and its metabolites. Test samples were either spiked with14C-D4or obtained from animals administered14C-D4via various exposure routes (inhalation, iv, etc.) and then subjected to THF extraction. Recoveries of D4and its unbound metabolites from plasma were essentially quantitative in three extractions. Even in a single extraction, the recoveries were >90%. Similar results were obtained for other matrices as well. Extraction efficiencies were measured as follows: lung, 98.2 ± 0.3%; liver, 95.4 ± 0.4%; fat, 99.4 ± 0.8%; urine, 98.1 ± 0.2%; feces, 94.1 ± 0.6%. HPLC profiles obtained before and after extraction were essentially identical, further confirming the suitability of THF as an extractant.
ISSN:0003-2697
1096-0309
DOI:10.1006/abio.1997.2475