Capillary electrophoresis of urinary normal and modified nucleosides of cancer patients

This paper gives a capillary electrophoretic method for the separation of 15 urinary normal and modified nucleosides from cancer patients in less than 40 min. A 500 mm×50 μm uncoated capillary column (437.5 mm to window) was used. The effects of the voltage and the sodium dodecyl sulfate (SDS) conce...

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Bibliographic Details
Published in:Journal of Chromatography A 1998-01, Vol.793 (2), p.341-347
Main Authors: Liebich, H.M, Xu, G, Di Stefano, C, Lehmann, R
Format: Article
Language:English
Subjects:
Biological and medical sciences
Chromatography, Affinity
Electrophoresis, Capillary - methods
Female
Humans
Investigative techniques, diagnostic techniques (general aspects)
Male
Medical sciences
Miscellaneous. Technology
Neoplasms - urine
Nucleosides
Nucleosides - urine
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Reproducibility of Results
Sodium Dodecyl Sulfate - pharmacology
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Summary:This paper gives a capillary electrophoretic method for the separation of 15 urinary normal and modified nucleosides from cancer patients in less than 40 min. A 500 mm×50 μm uncoated capillary column (437.5 mm to window) was used. The effects of the voltage and the sodium dodecyl sulfate (SDS) concentration in the buffer on the separation were studied. With reproducibilities of migration times better than 1.2% (R.S.D.) and determined concentrations better than 5–25%, depending on the concentrations of nucleosides in the urine, the analytical characteristics of the method were good. Using this developed method, the concentrations of 13 normal and modified nucleosides, extracted on a phenyl boronic acid affinity chromatography column, in 25 urines from patients of 14 kinds of cancer were determined. The levels (nmol/μmol creatinine) of modified nucleosides in urines from cancer patients were increased as compared with those in normal urines.
ISSN:0021-9673
DOI:10.1016/S0021-9673(97)00915-1