Lung function in premature lambs and rabbits treated with a recombinant SP-C surfactant

We evaluated the efficacy of a recombinant surfactant protein-C (rSP-C) surfactant in vitro and in vivo, using ventilated preterm lambs and rabbits. The surface tension measured with a Wilhelmy balance of the rSP-C surfactant was lower than the surface tension of natural sheep surfactant (containing...

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Bibliographic Details
Published in:American journal of respiratory and critical care medicine 1998-02, Vol.157 (2), p.553-559
Main Authors: DAVIS, A. J, JOBE, A. H, HÄFNER, D, IKEGAMI, M
Format: Article
Language:English
Subjects:
Animals
Animals, Newborn - physiology
Biological and medical sciences
Gestational Age
Lung - drug effects
Lung - physiology
Lung Compliance - drug effects
Medical sciences
Pharmacology. Drug treatments
Positive-Pressure Respiration
Proteolipids - pharmacology
Pulmonary Surfactants - pharmacology
Rabbits
Recombinant Proteins
Respiration, Artificial
Respiratory system
Sheep
Surface Tension
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Summary:We evaluated the efficacy of a recombinant surfactant protein-C (rSP-C) surfactant in vitro and in vivo, using ventilated preterm lambs and rabbits. The surface tension measured with a Wilhelmy balance of the rSP-C surfactant was lower than the surface tension of natural sheep surfactant (containing SP-B and SP-C). The rSP-C and natural surfactants were equivalently sensitive to inhibition by plasma. Over a 5-h study period, lambs treated with 100 mg/kg rSP-C and natural surfactants at birth had similar PCO2, lung mechanics, and FRC values. Indicators of injury also were not different. In preterm rabbits ventilated with 3 cm H2O positive end-expiratory pressure (PEEP), similar compliance responses were measured for the rSP-C and natural surfactant. Large-aggregate surfactants recovered from alveolar washes of the lambs treated with rSP-C or natural surfactant retained their function when tested in preterm rabbits. In conclusion, the rSP-C surfactant was effective in preterm animals with surfactant deficiency.
ISSN:1073-449X
1535-4970
DOI:10.1164/ajrccm.157.2.97-08019