Strategies for optimizing heterologous protein expression in Escherichia coli

The many advantages of Escherichia coli have ensured that it remains a valuable host for the efficient, cost-effective and high-level production of heterologous proteins. Here, we describe the current status of this prokaryotic expression system and focus on strategies designed to maximize the yield...

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Bibliographic Details
Published in:Trends in biotechnology (Regular ed.) 1998-02, Vol.16 (2), p.54-60
Main Authors: Hannig, Gerhard, Makrides, Savvas C
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Codon
Cytoplasm - metabolism
Escherichia coli
Escherichia coli - genetics
expression systems
Fermentation
Fundamental and applied biological sciences. Psychology
Gene expression
Genetic engineering
Genetic technics
heterologous proteins
Methods. Procedures. Technologies
Modification of gene expression level
Molecular and cellular biology
Molecular genetics
Promoter Regions, Genetic
promoters
recombinant proteins
Recombinant Proteins - biosynthesis
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Summary:The many advantages of Escherichia coli have ensured that it remains a valuable host for the efficient, cost-effective and high-level production of heterologous proteins. Here, we describe the current status of this prokaryotic expression system and focus on strategies designed to maximize the yields of recombinant proteins. Major challenges facing this expression system are also outlined.
ISSN:0167-7799
1879-3096
DOI:10.1016/S0167-7799(97)01155-4