Identification of the chicken anemia agent, reproduction of the disease, and serological survey in the United States

• Published in: Avian diseases 1990, Vol.34 (1), p.146-153
• Main Authors: Lucio, Benjamín, Schat, K. A., Shivaprasad, H. L.
• Format: Article
• Language: English
• Subjects: Anemia - microbiology; Anemia - veterinary; animal diseases; Animals; Antibodies; Antibodies, Viral - analysis; aves de corral; Bone marrow; Bone Marrow - pathology; Bursa of Fabricius - pathology; Centrifugation, Density Gradient; Chicken anemia virus; Chickens; encuestas; enfermedades de los animales; enquete; estados unidos de america; etats unis; Flocks; Hematocrit; Hematocrit - veterinary; immunologie; immunology; inmunologia; Lesions; Liver; Liver - pathology; Lymphocytes; maladie des animaux; Neutralization Tests; parvoviridae; pathologie; pathology; patologia; poultry; Poultry Diseases - microbiology; Poultry Diseases - pathology; sintomas; surveys; symptome; symptoms; Thymus Gland - pathology; usa; Virus Diseases - microbiology; Virus Diseases - pathology; Virus Diseases - veterinary; Viruses; Viruses - immunology; Viruses - isolation & purification; volaille
• ISSN: 0005-2086; 1938-4351
• DOI: 10.2307/1591346

An agent with antigenic, physicochemical, and pathological characteristics of chicken anemia agent (CAA) was isolated from broiler chickens and was designated chicken infectious anemia (CIA)-1. CIA-1 was resistant to chloroform treatment and passed through 50-nm-diameter-pore membranes. When inoculated in embryonally bursectomized and/or intact chickens, CIA-1 produced signs and lesions characteristic of CIA: low hematocrit values, pale bone marrow, thymic and bursal atrophy, and enlarged liver. Microscopic lesions were a reflection of macroscopic observations. When injected into 4-week-old chickens CIA-1 induced antibodies against the Cux-1 CAA isolate. In CsCl, CIA-1 had a density of 1.36 g/ml. Antibodies against CAA were found in breeder and commercial chicken flocks from Arkansas, Connecticut, Georgia, Kansas, Maine, Michigan, New York, and Pennsylvania. The age of these flocks ranged from 10 to 78 weeks. /// A partir de pollos de engorde, se aisló un agente con las características antigénicas, fisicoquímicas y patológicas del agente de la anemia del pollo. El agente, denominado CIA-1, es resistente al tratamiento con cloroformo y pasa las membranas con poros de 50 nm de diámetro. La inoculación en pollos intactos o bursectomizados durante su vida embrionaria, produjo signos y lesiones característicos del agente de la anemia del pollo: Valores de hematocrito bajos, medula ósea pálida, atrofia del timo y de la bolsa y hepatomegalia. Las lesiones microscópicas fueron un reflejo de las observaciones macroscópicas. En pollos de 4 semanas de edad, CIA-1 indujo anticuerpos contra la cepa Cux-1 de la anemia del pollo. La densidad de CIA-1 en CsCl fue de 1.36 g/ml. Se encontraron anticuerpos contra el agente de la anemia del pollo en parvadas de gallinas comerciales y en reproductoras de los estados de Arkansas, Connecticut, Georgia, Kansas, Maine, Michigan, New York y Pennsylvania. La edad de estos lotes era de 10 a 78 semanas.