Isolation and infusion of donor CD34+ bone marrow cells in cadaver kidney transplantation

Background. Infusion of donor bone marrow cells induces tolerance in allograft models. CD34+ stem cells present in human bone marrow could be endowed with tolerogenic properties. Methods. CD34+ stem cells were isolated from bone marrow extracted from vertebral bodies of cadaveric donors. Donor CD34+...

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Bibliographic Details
Published in:Nephrology, dialysis, transplantation dialysis, transplantation, 1998-01, Vol.13 (1), p.34-36
Main Authors: De Pauw, Luc, Abramowicz, Daniel, Donckier, Vincent, Kornreich, Anne, Destrée, Martine, Demoor, François, Andrien, Marc, Lambermont, Micheline, Goldman, Michel
Format: Article
Language:English
Subjects:
Adult
Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy
Antigens, CD34 - analysis
Biological and medical sciences
bone marrow
Bone marrow, stem cells transplantation. Graft versus host reaction
Cadaver
Hematopoietic Stem Cell Transplantation
Humans
Kidney Transplantation
Medical sciences
Middle Aged
stem cell
tolerance
Transfusions. Complications. Transfusion reactions. Cell and gene therapy
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Summary:Background. Infusion of donor bone marrow cells induces tolerance in allograft models. CD34+ stem cells present in human bone marrow could be endowed with tolerogenic properties. Methods. CD34+ stem cells were isolated from bone marrow extracted from vertebral bodies of cadaveric donors. Donor CD34+ cells (0.6–3.7 × 106/kg) were infused during surgery in 10 kidney transplant recipients receiving OKT3 as induction therapy. Chimerism was investigated using nested PCR for donor-specific HLA alleles. Results. The infusion of CD34+ stem cells was perfectly tolerated. Five patients remained free of acute rejection at follow-up, 47–325 days post-operatively. The five other patients underwent a single episode of corticosensitive acute rejection. Long-term chimerism was not induced in the seven patients investigated for the persistence of donor DNA. Conclusions. Infusion of donor CD34+ stem cells in kidney transplantation is safe. The clinical usefulness of the procedure remains to be established.
ISSN:0931-0509
1460-2385
DOI:10.1093/ndt/13.1.34